الفهرس | Only 14 pages are availabe for public view |
Abstract In this study we report the validation and establishment of a real time PCR assay using a TaqMan labeld probe for the detection of Mycoplasma ga/lisepticum. The MG rtPCR assay had the same specificity and was 10 folds more sensitive than cPCR. The detection limit of MG rtPCR was 1.6 CFU/ml, while it was 16 CFU/ml in cPCR. A total of 71 unvaccinated poultry flocks were examined, from which 16 (22.5%), 24 (33.85), 34 (47.9%) and 38 (53.5%) were positive by culture, ELlSA, cPCR and rtPCR respectively. from the 5 flocks vaccinated with killed MG vaccine, ELlSA showed positive results for 2 flocks, while the culture, cPCR and rtPCR results were negative for all of the 5 flocks. However, cPCR and rtPCR showed positive results for all of the 5 flocks vaccinated with live MG vaccine. While, only 3 flocks were positive by ELlSA and 4 flocks were positive by culture. In conclusion, the MG rtPCR was highly specific and sensitive than classical methods for detection of MG. |