الفهرس 
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Abstract
A total of 360 serum samples, 20 Buffy coat, 20 saliva and
20 epithelial tissues were collected from cattle from different localities of
Assiut Governorate (Arab El- Awamer, Sahel seleem, Dairoot, Veterinary
hospital, Aboteeg, Garb elbalad and Assiut) were used in this study and
subjected to different epidemiological, serological and virological studies
as summarized below:
1. The seroprevalence rate of FMDV NSP (non- structural
protein) antibodies in examined cattle at Assiut Governorate revealed that
the infection rate was 42.2% (Arab El- Awamer 47.4%, Sahel seleem
33.3%, Dairoot 18.2%, Veterinary hospital 25%, Aboteeg 20%, Garb elbalad
27.8% and Assiut 26.3%).
2. Clinical examination of infected animals infected with
FMDV revealed fever, excessive salivation, stomatitis, and lesions in feet
and in dairy cattle, vesicles detected on the udders.
3. Female cattle were more susceptible than males to infection
with FMD (female 65.8% (100/ 308) - males 34.2% (52/ 52)).
4. The infection rate was 88.2% in Old cattle (Above 4 years),
9.9% in Adult (Between 2 and 4 years) and 2% in Young (< 2 years).
5. Higher rate of infection was in non- hot months 47.03%
than hot months 33.06%.
6. Serum neutralization test was used for detection of FMDV
serotypes O and A antibodies in cattle sera, it was found that the total
positive sera for FMDV serotype O were 236 (65.6%) out of
360 examined sera. While it was found that the total positive
sera for FMDV serotype A were 214 (59.4%) out of 360
examined cattle sera.
7. Liquid phase blocking ELISA was used for detection of
FMDV serotypes O and A antibodies in cattle sera, it was found that the
total positive sera for FMDV serotype O were 270 ( 75%) out of 360
examined sera. While It was found that the total positive sera for FMDV
serotype A were 253 (70.3%) out of 360 examined sera.
8. PrioCHEKIT FMD-ELISA was used for detection of nonstructural
protein antibodies against FMDV. It was found that out of 360
examined sera 152 (42.2%) were positive for the presence of nonstructural
protein antibodies against FMDV detected in cattle.
9. Trials for Isolation of suspected FMDV from Buffy coat,
epithelial tissue, and saliva collected from infected cattle by three blind
serial passages on BHK21 cell culture. It was found that the positive
samples induced cytopathic effect after 24-48 hours
10. Indirect sandwich Enzyme Linked Immunosorbent Assay
(ELISA) was used for Identification and serotyping of field isolates of
suspected FMDV showed that type O and A were detected in the
examined samples by percentage of 27(67.5%) out of 40 and 23 (57.5%)
out of 40 respectively.
11. All positive epithelial tissue samples for isolation on tissue
culture were also positive for RT-PCR and Real- time PCR