الفهرس | Only 14 pages are availabe for public view |
Abstract Sugar beet can be attacked by various diseases which affect its quantity and quality. Damping-off of seedlings and root rot diseases were considered among the most destructive diseases which are caused by many serious soil borne pathogens namely, Sclerotium rolfsii Sacc. The main goals of the present study to investigate the following point: Under lab. Conditions: 1. The effect of some metals and herbicide (Topik) on linear growth of antagonistic fungi Trichoderma harzianum, T. hamatum and the pathogenic fungus Sclerotium rolfsii. 2. The effect of IC50 of the tested metals on linear growth of the above mentioned fungi. 3. Effect of some metals and the herbicide on sclrotia formation of Sclerotium rolfsii. 4. Effect of some metals and the Topik herbicide on the antagonism between either Trichoderma harzianum or T. hamatum against Sclerotium rolfsii. 5. Testing the biosaftey of the biocontrol agent. Under field conditions: 1. Studying the effect of cupper and the herbicide Topik on sugar-beet damping disease caused by Sclerotium rolfsii. 2. Studying the antagonism between Trichoderma harzianum or T. hamatum on controlling sugar-beet damping disease. 3. Studying the combined effect of copper and the herbicide Topik on sugar-beet damping disease .4. Effect of the previous treatments on yield components of sugarbeet, weight of root under stress of cupper and/or herbicide. The obtained results could be summarized as follow: 1- Sclerotium rolfsii followed by Fusarium solani then R. solani were the most prevalent fungi giving the highest frequencies of damping off and root-rot of sugar beet 2. T. harizianum and T. hamatum were identified as the antagonistic fungi isolated from the rhizosphere of healthy sugar beet growing in the farm of Gemmeiza Research station. 3- Both of the Trichoderma isolates did not exudates pectinase enzyme and proved to be non-pathogenic and could be used safety as a bio-control agents. 4-Under lab. Conditions, All the metals used showed toxicity to the dual growth of the tested fungi, nickel followed by cobalt ions were the most affective metals one on the pathogenic fungus S. rolfsii. While the copper ion was the most affective one on the radial growth of the antagonistic fungi followed by zinc ion . |