الفهرس 
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Abstract
Traditional methods of sterilization have passive side effects
on the treated cells, medical tools, or tissues because its
temperature raises effect, so this thesis focuses on the
possibilities of applying the plasma in biomedical applications.
For a reliable study of the biological effects it is necessary to have
stable and reproducible plasma. Thus, the design of the plasma
sources is an important part of the study.
Utilizing atmospheric non-thermal plasma to achieve
sterilization is an alternative to conventional sterilization
techniques. Plasma is an efficient source of various radicals and
UV irradiation capable of bacterial decontamination, while it
operates at room temperature and does not cause bulk
destruction of the treated tissue. Plasma induces local changes to
cells in a non-thermal and non-contact way; the plasma can
easily penetrate the cavities, which are not possible with lasers.
Also the use of plasmas is relatively cheap compared to the use
of lasers. Working area of the atmospheric plasma can be small.
The main effect for cells in culture was detachment of cells
without necrosis.
In particular, there are three tasks to fulfill:
1. The profound understanding of processes and physical
plasma parameters; the electrical characteristics of the plasma
indicating the variation of the discharge current, voltage, power,
power factor, flow rate, distance from the central electrode to the
treated sample, and gas temperature.
2. Comparing the plasma efficacy of three different plasma
sources which are RF plasma needle, RF plasma pencil, and LF
plasma jet in the sterilization process.
3. The characterization of special biologic effects; by plotting
the survival curve, which is a relation between colony forming
units per unit volume ”CFUs” against plasma exposure time.
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