الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Kinesin motor proteins play important roles in Trypanosoma brucei and other eukaryotic cells including the transportation of cargos for instance, organelles and chromosomes in addition to the pivotal role in the chromosomes segregation and intraflagellar transport. This study was performed on three kinesins aiming to subcellular localize and function characterize namely, TbKif5, TbKif22 and TbKif37 (geneDB access number: Tb927.7.5650), (geneDB access number: Tb927.7.7120) and (geneDB access number: Tb927.8.2630) respectively. This study utilized various approaches and techniques to check the effect of these kinesins on the cell cycle progression and the cell growth of Trypanosoma brucei procyclic form.
Immunolocalization of these kinesins were detected by the immunofluorescence microscope. Of the three kinesins (TbKif5) was found to stain the cell body excluding the nucleus and flagellum, while the second (TbKif22) was found on the whole cell body cytoskeleton without any traces in the nucleus and flagellum with detection of the signal of this protein on the tip of the posterior end of the cell and finally the TbKif37 which is subcellular localized on the tip of the posterior end of the cell where the plus end of microtubules are found without staining of any part of the remaining body of T. brucei procyclic form.
The function characterization of these kinesins was performed through depleting them from the cells by RNAi technique to investigate the growth phenotype and any possible morphological alteration appear in response to their depletion. However, the RNAi created against TbKif5 and TbKif22 as a depletion method, no growth phenotype or morphological changes were observed and this is not uncommon. While using overexpression experiment as another choice to study gene function revealed no effect on TbKif22 but gives a strong evident effect on TbKik5 since, the overexpressed cells displayed cell shape abnormalities with multinucleated cells. The last kinesin, TbKif 37 experimented in this study showed growth impairments, cell cycle progression defects and a morphological phenotype exemplified in generating cells of multiple nuclei and multiple kinetoplasts.