الفهرس 
Non Hodgkin LymphomasOnly 14 pages are availabe for public view
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Abstract
N
HL comprises an extremely heterogeneous group of clonal LPDs that might be derived from either B-cell or T/NK-cell lineages. Within this broad term of NHL, numerous disease entities with variable clinical behavior and diverse molecular features were included.
The identification of genes involved in the pathogenesis of NHL has yielded a practical benefit of allowing the development of highly specific and sensitive cytogenetic and molecular assays. These assays have greatly improved lymphoma diagnosis through resolving diagnostic ambiguities brought about by the more traditional classification methods; which were subsequently refined following the identification of non-random chromosomal aberrations specific to each lymphoma subtype.
Molecular pathogenesis of NHL represents a complex process involving the accumulation of multiple genetic lesions which include: the activation of proto-oncogens such as BCL1, BCL2, BCL6, c-MYC by chromosomal translocation, as well as inactivation of tumor suppressor genes such as TP53 by chromosomal deletion or mutation.
In light of these data, the present study aimed to detection of Immunoglobulin heavy chain (IgH) gene rearrangement by FISH technique in paraffin embedded bone marrow trephine and lymph node biopsies. Moreover, the presence of IgH chain gene rearrangement will be correlated to the standard prognostic factors of NHL.
The present study was carried out on 50 newly diagnosed adults with NHL. Their age ranged from 40 to 69 years with mean & SD (53.02 ± 7.47 years). Thirty seven of them were males and 13 were females with male to female ratio of 2.9.
The study included 26 DLBCL patients 21(80.8%) of them were males & 5(19.2%) females with male to female ratio 4.2. Their age ranged from (41-69 years) with mean and SD (52.88±8.05 years) , 21 FL patients 13/21 (61.9%) of them were males and 8/21 (38.1%) females with male to female ratio of 1.6. Their age ranged from (40 – 67 years) with mean and SD (52.76 ± 7.35) and 3 ML patients all of them were males. Their age ranged from (54 – 57 years) with mean ± SD of 56 ± 1.73.
In the present study, IgH gene rearrangement were detected by FISH technique in lymph node sections of FL patients in 16/21 (76%) and in one patient by trephine biopsy regarding DLBCL IgH gene rearrangement was detected in lymph node sections of 17/26(65.4%) patients and in two patients by trephine biopsy and Regarding MCL IgH gene rearrangement was detected in all patients (3/3) both by lymph node and trephine biopsies.
It was found that 14q rearrangement was significantly associated with splenomegaly, staging, higher TLC, PB lymphocytic count, LDH and Hb levels.
In conclusion, this study demonstrates the increasing importance of detailed cytogenetic analysis of NHL cases and stresses the superiority of FISH technique done on L.N over FISH technique on trephine biopsies in detecting targeted aberrations. Furthermore, IgH chain rearrangement (14q32) was found to be present in a considerable number of B-NHL cases on LN over trephine biopsy.
Immunoglobulin Heavy Chain Gene Rearrangement in B-cell Non-Hodgkin Lymphoma Using Fluorescence In Situ Hybridization Technique.