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العنوان
BIOTECHNOLOGICAL STUDIES ON THE
INSECTIVOROUS PLANTS AND THEIR EFFECT
ON THE GROWTH OF DATE PALM USING TISSUE
CULTURE TECHNIQUES/
المؤلف
Ali, Ibrahim Mohamed Shams El Din
هيئة الاعداد
باحث / Ibrahim Mohamed Shams El Din Ali
مشرف / Ibrahim Abd Elmaksoud Ibrahim
مشرف / Hamdy Ahmed Abduallah Emara
مشرف / Abd El Moneem Abd El Wadoud Mohamed El Banna
الموضوع
النبات - فسيولوجيا
تاريخ النشر
2011 .
عدد الصفحات
700 mg :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Biochemistry, Genetics and Molecular Biology (miscellaneous)
تاريخ الإجازة
1/1/2011
مكان الإجازة
اتحاد مكتبات الجامعات المصرية - Plant Biotechnology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Carnivorous or insectivorous plants belong to several botanical families,
the most important of them is Droseraceae, which includes Drosera plants.
Some economic substances are extracted from Drosera. Tissue culture
technique provides the best way to obtain as high and clean quantity as
possible of the biomass needed to obtain these substances. This study
aimed to propagate the Drosera capensis in vitro and evaluate its content
from phytohormones and total amino acids, indoles and phenols. Also
studying the effect of Drosera capensis leaf and root extracts as plant
growth substances on in vitro growth of Phoenix dactylifera cv.
Bartamouda as one of the most important crops. And studying the effect of
Drosera capensis residue on larva of red palm weevil. Shoots were visible
on leaf explants, apparently forming directly on leaf surfaces without
intermediate callus. The best results of shoot number (13.8 shoots per
explant) and shoot length (2.93 cm) were obtained at 0.05 mg L-1 BA
compared with the control, BA-free media, observed 2.8 shoots per explant
and 2.27 cm in length. MS basal medium supplemented with 1.0 mg L-1
IBA achieved the best root formation where the root number was 47.3 per
plant. Using of ½ MS medium supplemented with NAA at 0.25 mg L-1 and
BA at 0.3 mg L-1 gave rise to biggest callus weight. The amounts of
phytohormones in Drosera capensis plant (mg 100g-1 fresh weight) were
as following: Indole acetic acid in leaf was 2.055 mg, while in case of root
was 2.291 mg. Zeatine in leaf was 1.609 mg, while in case of root was
0.418 mg. Other Cytokinins in leaf was 18.791 mg, while in case of root
was 1.003 mg. Gibberellic acid in leaf was 70.938 mg, while in case of
root was 86.59 mg. Abscissic acid in leaf was 0.500 mg, while in case of
root was 0.158 mg. The amount (mg 100g-1 fresh weight) of total amino
acids in leaves was 200 mg, while in case of roots were 100 mg. The
amount of total indoles in leaves was 17 mg; while in case of roots was 11
mg. The amount of total phenols in leaves was 0.05 mg; while in case of
root was 0.02 mg. Concentrations of the extract of Drosera capensis leaves
and roots were applied at different ratios in in vitro experiments of date
palm cv. Bartamouda. The results revealed that Drosera capensis root
extract had a significant effect on fresh weight of date palm embryogeneic
callus as the best result (4.63g) was observed with using Drosera capensis
root extract at 3.0ml L-1 (0.042g residue). Using of Drosera capensis root
extract at 0.05ml L-1 (0.0007g residue) gave rise to higher number of
mature embryos. The highest significant shoot number (21 shoots) of date
palm was obtained with using 1.0ml L-1 (0.01g residue) Drosera capensis
leaf extract. Also the length of date palm shoots increased significantly by
using the same concentration of Drosera capensis leaf extract and reached
3.3cm. In rooting stage, the best result was obtained with the use of
Drosera capensis root extract at 1.0ml L−1 (0.014g residue). In
acclimatization stage, the best result was obtained with the use of Drosera
capensis root extract at 1.0ml L−1 (0.014g residue). Finally, in vitro date
palm cultivation can be achieved with MS medium supplemented with
Drosera capensis extract as a source of phytohormones at different
micropropagation stages. The residue of Drosera capensis plants at
different concentrations (0.0, 50.0, 100. 500.0 mg per liter) had been given
to fully developed larvae of red palm weevil (Rhynchophorus ferrugineus
Oliv.) through their feeding diet. Larvae were obtained from the field and
were maintained on the stems of sugarcane prior to mass rearing, artificial
diet, which was formulated from sucrose, molasses, potatoes and agar. The
residue of Drosera capensis had toxicological effects on R. ferrugineus
larvae. The lethal action of Drosera capensis residue had appeared clearly
at 500 mg L-1 where the lethal percentage of red palm weevil larva was
65% after ten days.
Key words: Drosera capensis; Shoot multiplication; Benzyl
adenine; Rooting stage; Indole butyric acid; Drosera capensis extract;
Phytohormones; In vitro; date palm cv. Bartamouda; Embryogeneic callus;
Mature embryos; Rhynchophorus ferrugineus