الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 210 |  |  |
| | | from | 210 | | |
Abstract
In this work, important pharmaceutical compounds were analysed in pure form or in their pharmaceutical formulations using either ion pair complex formation or charge transfer reaction or redox reactions.
It is of great importance to develop sensitive, precise and accurate methods for determination of the investigated drugs, either in pure form or in the pharmaceutical formulations with low cost depending upon the availability of chemicals and equipments.
The investigation includes the use of certain spectrophotometric, spectrofluorimetric techniques.
The thesis consists of four parts in addition to detection of bilins produced by cleavage of the thiol linkage between bilin and protein in phycobiliproteins present in cyanobacteria.
Part (I):
This part demonstrates a theoretical basic background review on the official and other methods of analysis for the selected drugs and their chemical structure and pharmacological action.
Part (II):
This part is based on ion pair reactions, it includes three sections:
Section (1):
This section describes the Spectrofluorimetric determination of fexofenadine HCl, moxepril HCl and etodolac using europium chloride in their pure form or in their pharmaceutical formulations.
Section (2):
This section is concerned with studying the spectrophotometric determination of clemastine hydrogen fumarate, desloratadine, losartan potassium and moxepril HCl using eosin.
Results obtained showed good agreement with those obtained by the official and reported methods.
Section (3):
This section describes the Spectrophotometric determination of clemastine hydrogen fumarate, fexofenadine HCl and moxepril HCl using chromatrope 2 R.
There was no significant difference between the proposed and the official or reported methods.
PART (III):
This section describes the spectrophotometric and spectrofluorimetric determination of loratadine, ramipril, clemastine and losartan through charge transfer complex formation with NBD-Cl.
The proposed method was simple, accurate and in good agreement with the official and reported methods.
PART (IV):
This part describes the application of redox reactions in analysis of the selected drugs, it includes three sections:
Section (1):
This section deals with using cerium (IV) for the spectrophotometric and spectrofluorimetric determination of etodolac, desloratadine and moxepril
HCl in their pure form or in their pharmaceutical formulations. The proposed methods were simple, accurate and sensitive.
Section (2):
This section describes using alkaline KMnO4 for the spectrophotometric determination of moxepril HCl, thiocolchicoside, fexofenadine HCl, desloratadine and etodolac.
The proposed method was simple, accurate and showed good agreement with results obtained by the reported methods.
Section (3):
This section is dealing with the Spectrophotometric determination of etodolac, thiocolchicoside using ammonium molybdate.
The proposed method was simple, accurate and results obtained showed good agreement with those obtained by the reported methods.
PART (V):
Detection of the phycobiliproteins expressed by cyanobacteria is facilitated by the highly-fluorescent phycobilins (or more simply,bilins) that are covalently linked to the protein via cysteinyl residues. Various thiol-cleaving reagents such as dithiothreitol (DTT) and tris-(2-carboxyethyl) phosphine (TCEP) have been employed to remove the bilins from the proteins, and the establishments of CE / LIF conditions suitable for bilin analysis have been developed.