الفهرس 
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Abstract
This study was carried-out on sixty normal Oreochromis niloticus fish with average body weight (150± 10g) under natural conditions.fish were starved for 24 hrs and the mucus was collected from each fish from different localities in different interval period on differrent seasons of the year.The pooled mucus collected from skin, pharyngeal, intestine The mucus collected from skin cover slide was passed with same direction of the scales. The mucus collected from pharyngeal and intestine In both location the fish must be scarified first for the pharyngeal mucus it collected by imagination of area of gills. In case of mucus samples collected from intestine, small incision was made above any part of gastrointestinal tract then complete open the whole gastrointestinal tract for scraping of mucus using cover slide were extracted separately with acidic organic. Mucus separated and the mucus preparated for measuring mucus lysozomal activity, peroxidase activity and immunoglobulin for identification the relation ships between them and their importance of them in prevention of the diseases incidence in fish under natural conditions. These carried out at different seasons of the year to know relation ship between the peroxidase, lysozyme and immunoglobulin in and seasons of the year.
This study concluded that:
1-Identification of the importance of peroxidase, lysozyme and the immunoglobulin in different mucus (skin, pharyngeal and intestinal) in prevention of the diseases incidence in fish under natural conditions.
2-Identification of the relationships between the peroxidase, lysozyme and immunoglobulin in different fish mucus and in different seasons of the year.
-Important results:
1-The mucus of the fish of either skin, pharyngeal or intestinal play an important role in natural immunity of the fish against different bacterial pathogens affecting fish.
2- The peroxidase level showed higher level in the skin followed by pharyngeal mucus and the lowest level observed in intestinl mucus. The peroxidase level showed a higher level in autumn season but the level in the other seasons is nearly equal in its peroxidase level.
3-There is a positive relationship between the level of peroxidase in intestinal mucus and pharyngeal mucus . While the increase in level of peroxidase enzyme in skin mucus causes decrease in its level in pharyngeal and intestinal mucus..
4- The lysozyme level showed higher level in intestinal mucus, that is very high compared to the lysozomal enzyme in the in the skin which showed the lowest level and the pharyngeal mucus which showed the second level in its content in lysozyme.
5-There is a positive relationship between the level of lysozyme enzyme in intestinal mucus and pharyngeal mucus and between the level in the intestinal mucus and skin mucus. While the increase in level of peroxidase enzyme in skin mucus causes decrease in its level in pharyngeal mucus and intestinal mucus.
6-The globulin level was very high in skin mucus which came in the first degree before both pharyngeal and intestinal mucus. On the other hand the globulin level in both pharyngeal and intestinal mucus were similarly equal but the intestinal globulin was slightly higher than pharyngeal globulin. The globulin level showed higher level in summer , followed by spring and the lower seasons were winter and finally the autumn season which show the lowest level.
7-There is a positive relationship between the level of globulin in intestinal mucus and pharyngeal mucus and between the level in the skin mucus and pharyngeal mucus .