الفهرس 
PSEUDOMONAS SPECIESOnly 14 pages are availabe for public view
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Abstract
During the past decade, nosocomial outbreaks of Pseudomonas spp. have been described with increasing frequency, occurring mostly in surgical wards, intensive care units and internal medicine wards. Epidemic strains of Pseudomonas spp are often resistant to several antimicrobial drugs, which reduce treatment effectiveness.
Pseudomonas spp. is an opportunistic pathogen. It causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and also causes other blood infections. It’s the most common cause of burn and external ear infections, and is the most frequent colonizer of medical devices (e.g. catheters). Pseudomonas can in rare circumstances cause community acquired pneumonias, as well as ventilator-associated pneumonias, being one of the most common agents isolated in several studies.
During our study we collected 150 Pseudomonas spp.which was received at Ain Shams University Microbiology Laboratory. Most of these isolates were from wound swab: 64% (96/150), sputum: 14.7% (22/150) and urine: 8% (12/150).
In this study most of the Pseudomonas spp. was isolated from surgical unit (48.7%), internal medicine wards (24%) and ICU (22.6%).
All isolates of Pseudomonas were subjected to routine culture on blood agar and MacConkey agar media for 24 to 48 hours incubation at 37oC. Identification of the isolates was done using Gram stain morphology, conventional biochemical tests and antibiotic susceptibility test by disc diffusion method. We found the most effective antibiotic against Pseudomonas was carbapenems (74.6%), pipracillin/tazobactam (72%), cefepime (48%), amikin (39.4%), ciprofloxacin and cefotaxime (32.7%) and fortum (6%).
38 out of 150 isolates (25.4%) were carbapenems resistant. Most of these isolates were from wound: (65.7%), sputum: (21%) and blood: (7.8%) and most of these isolates were from surgical units (55.2%), ICU (34.2%) and internal medicine (7.8%).
Carbapenems resistant isolates were subjected to detection of blaIMP genes using polymerase chain reaction (PCR). 36.8% expressed blaIMP gene.
In our study we found that the most effective antibiotics against carbapenems resistance and blaIMP positive strains were pipracillin/ tazobactam and fourth generation cephalosporins.
We also found that Pseudomonas is highly resistant to fortum it is nearly 100% resistant. However Pseudomonas spp. which express blaIMP gene were also highly resistant to qunilones and aminoglycosides.