الفهرس 
Suitable media for plant propagation (Hyoscyamus muticusOnly 14 pages are availabe for public view
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Abstract
Nabil Ebeid Hanna Hanna Bebawy: Selection of Some Medicinal Plant Strains for Salt Tolerance Through Tissue Culture Technique. Unpublished Ph.D. Thesis, Department of Horticulture, Faculty of Agriculture, Ain Shams University, 2009.
Response of three different medicinal plants. Egyptian henbane (Hyoscyamus muticus L.), Atropa ( Atropa belladonna L.) and Madagascar periwinkle (Catharanthus roseus) to various levels of salt were investigated.
Two tolerant clones to salt were selected from henbane callus cultured on the selected medium which contained 6000 and 8000 ppm of salts, respectively. Tolerant clones were capable of proliferating and growing a medium contained the same salt concentrations and supplemented with 1.5 ppm BA. Vegetative characters were significantly enhanced in general at the lower salt concentration (6000 ppm) especially with the medium containing 0.12 g/l KH2PO4 +4 ppm kinetin + 0.5 ppm IBA. Using MS medium at its half strength with 1ppm NAA was the best for henbane root growth at 6000ppm level of salt. Survival percentage reached up to 28.00 % for clone A (selected at 6000 ppm) and 32.02 % for clone B(selected at 8000ppm) with a total alkaloids percentage of 0.90 and 0.98 %, respectively. Electrophoretic pattern of the resulted henbane plantlets of both clones and control plants cleared remarkable differences among molecular weights of the resulted bands which proved the presence of genetic differences among the selected clones.
Also, two tolerant clones to salt were selected from Atropa callus cultured on the selected medium which contained 4000 and 6000 ppm of salts, respectively. Tolerant clones were capable of proliferating and growing on the medium which contained (MS media + 1 ppm BA + 1ppm IBA + 2.5 ppm GA). Vegetative characters were significantly enhanced in general at the lower salt concentration (4000 ppm) especially with the media supplemented with 1 ppm IBA + 2.5 ppm GA.
. Using MS medium at its half strength + 1 or 5 ppm IBA was the best for Atropa root growth at 4000 ppm level of salt. Survival percentage reached up to 21.44 % for clone A (selected at 4000ppm) and 11.50 % for clone B (selected at 6000ppm) with a total alkaloids percentage of 0.70 and 0.25 %, respectively. Electrophoretic pattern of the resulted plantlets of both clones and control plants has not cleared remarkable differences among molecular weights of the resulted bands which may suggest that the salt tolerance may be due to physiological aspects rather than genetic differences.
Similarly, two tolerant clones to salt were selected from Catharanthus roseus callus cultured on the selected medium which contained 2000 and 4000 ppm salts, respectively. Tolerant clones were capable of growing on the medium which contained MS + 1ppm BA + either 1ppm IBA or 3 ppm IBA.
Vegetative characters were significantly enhanced in general at the lower salt concentration (2000 ppm) especially with the MS medium supplemented with 2ppm BA + 1ppm NAA +1ppm IBA.
Using MS medium at its half strength + 1ppm IBA was the best for Madagascar periwinkle root growth at 2000 ppm of salt.
Survival percentage reached up to 41.15 % for clone A (selected at 2000ppm) and 11.22 % for clone B (selected at 4000ppm) with a total alkaloids percentage of 0.0023 µg and 0.0012 µg, respectively. Electrophoretic pattern of the resulted plantlets of both clones and control plants has not cleared remarkable differences among molecular weights of the resulted bands which may suggest that the salt tolerance may be due to physiological aspects rather than genetic differences.
Key words: Salt tolerant clones - Tissue culture technique - Hyoscyamus muticus- Atropa belladonna- Catharanthus roseus.