الفهرس 
PREFACEOnly 14 pages are availabe for public view
 |  | from | 156 |  |  |
| | | from | 156 | | |
Abstract
AlSafa Hassan Mohamed, Molecular Taxonomy of Some Selected Taxa of Subfamily Mimosoideae. PhD of Science in Botany (Plant Taxonomy), Department of Botany, Faculty of Science, Ain Shams University, 2009.
The Fabaceae “Leguminosae” is one of the most important and large families of angiosperms. The Fabaceae comprises three subfamilies Mimosoideae, Caesalpiniodeae and Papillinoideae. Mimosoideae compromises approximately 50 to 60 genera distributed throughout tropical, subtropical and warm temperate regions of the world. Almost two-third of the known species fall into three genera Acacia with 1200, Mimosa with 400 to 500 and Inga with 350 to 400.
In the present study, taxonomic and genetic relationships were examined using Nei coefficient to compute the similarity matrices and applying the Unweighted Pair Group Method using Arithmetic (UPGAM) average to plot dendograms to separate the twelve species of three tribes of Mimosoideae. The first dendogram was based on the information derived from morphological and pollen grain characters; the second dendogram was based on the molecular criteria revealed by RAPD-PCR, ISSR and AFLP profiles. For morphological and pollen grain characters, the observations led to various conclusions of taxonomical importance. This was shown by an artificial key suggested for the species delimitation.
As regards the molecular study, some difficulties were met with when the DNA isolation was tried. A method for overcoming this difficulties were presented, stressing on a present priority to separate the DNA from already growing Acacia trees, but not from the germinating seeds. At the same, the advantage of the molecular approach in solving taxonomic problems was also presented.
RAPD analysis was done utilizing twenty three 10-mer random primers and ISSR was applied utilizing seven primers and four combinations of AFLP; and all resulted in the occurrence of some molecular genetic markers delimited the individual species.
The overall work culminated in a discussion of the taxonomic position and the inter-specific relationships of the studied species based on different criteria which made the conclusion more acceptable.
Keywords: Mimosoideae, Legum tree, Acacia tree, RAPD-PCR, Genetic differentiation, Fingerprints, Genetic relationships.