الفهرس 
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Abstract
All collected samples were cultured and identified in Assiut University Mycology Center (AUMC). Specimens were cultured on
sabouraud’s dextrose agar media (SDA) and Malt extract agar medium
(MEA) for isolation of fungi. Czapek yeast extract agar media (CYA) and
Potato sucrose agar media (PSA) used for identification of mould.
Identification based on the morphology of the colony, the rate of growth
and microscopic morphology of the isolates. Identification of Candida
species was confirmed by growing yeast on CHROMagar TM Candida.
ELISA test was carried in Molecular Biology Research Unit,
Assiut University for detection of Aspergillus IgG.
A total of 100 cattle suffered from reproductive disorders were
selected from Abnoub El-Hamam farm, Assiut Governorate. The
incidence of mycotic infection in examined 100 cows’ vaginal swab was
68%. Incidence of Aspergillus species was 41%. While the incidence of
Candida species was 17%. The percentage of mixed infection with
Aspergillus and Candida species was 17.6% out of 68 positive cows’
vaginal swab samples.
The incidence of mycotic infection in examined 100 subclinical
mastitic bovine milk samples was 89 %. The incidence of Aspergillus and
Candida species were 36% and 69% respectively. Mixed infection with
Candida and Aspergillus spp. was detected in 28% of positive bovine
milk samples. The infection rate in Abnoub El-Hamam and Faculty of
Agriculture Farm were 94.4% and 86% respectively.
A total 100 lung samples showing macroscopic lesion (abscess,
anthracosis, congestion and area of hepatizition) were collected from
slaughter houses in Assiut Governorate. The incidence of mycotic
infection in 100 examined bovine lung samples was 91%. The incidence
of Aspergillus spp. and Candida spp. in examined lung samples was 81%
and 27% respectively. Mycotic infection was recorded in different
localities as 100%, 92%, 87.1% and 83.3% in Mosha, El-Mateaa,
Manqabad and Abnoub localitied respectively.
In the present study, a total of 44 and 18 mould species were
isolated from soil and DROPping samples collected from different localities
in Assiut Governorate respectively.
The incidence of pulmonary fungal infection in 100 examined
sputum samples from patient admitted in Chest Hospital, Assiut
Governorate was 97%. The incidence of Aspergillosis in human patients
was 88%. The higher incidence was recorded among male patients
(89.4%) comparing with female patients (85.3%). The infection rate was
directly proportional with age. 36.4 % of Aspergillus positive patient
were suffered from T.B., 26.1% from bronchial asthma, 22.7 % from
bronchitis & chest pain and 14.8% from pneumonia. Incidence of
Aspergillus fumigatus by using culture method and ELISA IgG were 16
% and14% respectively.
The incidence of Candidiasis was 58% out of 100 examined patient
sputum samples. The incidence of infection among male and female
patients was 59.1 %, and 55.9 % respectively. The intensification of the
infection (74.4%) among the age group 31- 50 years was discussed. This
study clarified that 38% of Candida positive patient were suffered from
bronchial asthma, 24.1% from T.B., 20.7 % from bronchitis & chest pain
and 17.2 % from pneumonia.
In our study, 11 CSF samples were collected from patients
admitted to Assiut University Hospitals, Neurology Department. Candida
tropicalis was isolated from two patients who were resistant to usual
treatment.
It is advisable that close co-operation should be established among
veterinarians, physicians and medical mycologists to resolve the public
health problems involved with the mycoses