الفهرس | Only 14 pages are availabe for public view |
Abstract Ibrahim Hussein Osman Amer: Seed Potato Production Using Microtuber. Unpublished M.Sc. Thesis, Arid Land Agricultural Graduate Studies and Research Institute, Faculty of Agriculture, Ain Shams University, 2010. br This study was carried out at the tissue culture laboratory of Arid Land, Agriculture Research Unit, The Faculty of Agriculture, Ain Shams University, during the period -#102;-#114;-#111;-#109; 2006-2009 . The objective of this work was to study the effect of some factors affecting in vitro microtuberization of potato as effect of cytokinins, growth retardants and sucrose concentrations. The second objective of this research was to know the best cultivar to produce shoots -#102;-#114;-#111;-#109; microtubers and after that produce minitubers. Three concentrations of Kin and BA (1, 2 and 3 mg/l) were used, also three concentrations of Paclobutrazol and Uniconazol (1, 2 and 3 mg/l) were used in this experiment. Likewise, some concentrations of sucrose were used at 20, 40, 60, 80, and 100 g/l to know the best concentration of sucrose to enhance microtubers formation. The results showed that the highest percentage of tuberization was obtained -#102;-#114;-#111;-#109; cv. ”Draga” grown on modified MS medium supplemented with 3 mg/l Kin or BA. The same results with both fresh weight of microtubers and its number of microtubers / propagule. With regard to growth retardants, the results showed also that the highest percentage of tuberization was obtained -#102;-#114;-#111;-#109; cv.”Draga” grown on MS medium with 1 mg/l Paclobutrazol. The same results with both fresh weight of microtubers and its number of microtubers / propagule. The highest percentage of tuberization was obtained -#102;-#114;-#111;-#109; cv. ”Draga” when cultured on MS medium supplemented with 80 g/l sucrose. On the contrary, cvs. ”Draga and Cara” produced the lowest values related to fresh weight of microtubers , its number of microtubers / propagule and percentage of microtuberization without significant difference on the control medium. 80g/l sucrose gave the highest values of weight of microtubers / propagule, weight of microtubers and number of microtubers / propagule and percentage of microtuberization. Significant differences appeared between two cultivars in the yield characteristics. That cv. ”Draga” had the highest survival percentage of microtubers, plant height, stem, leaves number per plant. Significant differences appeared between two cultivars in the yield characteristics. That cv. ”Draga” had highest fresh weight / propagule, total yield and number of minitubers / propagule. br Key word: br in vitro, Potato, Kinetin, Benzyl adenine, Paclobutrazol, Uniconazol, sucrose, minituber. |