الفهرس 
SALMONELLOSISOnly 14 pages are availabe for public view
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Abstract
Typhoid fever continues to be a global health problem especially in tropics and subtropics. Early and accurate diagnosis is necessary for prompt and effective treatment. One has to rely on serological diagnosis since many diagnostic laboratories in developing countries do not have facilities for blood culture. Widal test is the mainstay in the diagnosis of typhoid fever in most laboratories but it has drawbacks. The present study was planned to study the role of a dot enzyme immunosassay (Typhidot-M) in the diagnosis of typhoid fever, and to evaluate its sensitivity and specificity in relation to other serologally and cultural tests in the diagnosis of acute typhoid fever in children. This study was carried out on 90 patients, 60 patients with clinical signs and symptoms of acute typhoid fever as (headache, abdominal pain, vomiting, diarrhea and lethargy) attending the out-patients clinics of Benha fever hospital, through-out the period from May, 2011 to September, 2011. They were 29 males and 31 females with mean age of (10.3± 1.8) years, representing the study group (group I). Thirty healthy age and sex matched children (16 males and 14 females) were selected as a control group (group II). All patients were subjected to full history taking, detailed clinical examination and laboratory investigations including: CBC, blood culture for salmonella typhi, Widal test and Typhidot-M test. from the Investigations which held in this study we had the following results: As regard to complete blood picture (CBC) there are insignificant differences between cases and control in either Hemoglobin level, platelets count, monocytic, eosinophilic and basophilic count while there are significant increase in total leucocytic count (TLC), increase lymphocytic count and decrease neutrophlic count. As regard to blood culture result it found that, out of the sixty clinically suspected as typhoid fever cases, S. typhi was isolated from blood culture in 33 cases (55%), and the remaining 27 cases (45 %) were blood culture negative.
Out of the sixty clinically suspected as typhoid fever cases, Widal test was positive in 26 cases (44.3%), while Typhidot-M test was positive in 49 cases (81.7%).
Out of 33 blood culture positive Widal test was positive in 15 cases(45.5%), while Typhidot-M test was positive in 31cases(93.9%).
Typhidot-M test has sensitivity of 93.9%, specificity of 83.6 %, positive and negative predictive value of %63.3 and 81.8 as compared to widal test which had sensitivity of 45.5 %, specificity of 59.3 %, positive and negative predictive value of 57.7 % and 47.1 %.
We concluded that Typhidot-M test is sensitive and rapid test and can be used as a valid tool in the early diagnosis of typhoid fever.