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Abstract In the present study, we have evaluated the prophylactic activities of silymarin against liver cells damage due carcinogenic effect of DENA in rats at molecular level, through the evaluation of its effects on the expression of rnRNA of SOD, CAT, p53, and COX-2. Sixty four male Albino rats were used in the study. Their Wight ranged from 160-180 grams. The rats were divided into 6 groups: Control group, DENA Group (rats injected with a single i.p. dose of DENA (200mg/kg), two weeks later they were injected i.p. with CC14 (2 mL/kg) three times ( a dose / week) to induce HCC), two pretreated groups with 40 and 80 mg/kg bw SMN, and two post-treated groups with 40 and 80 mg/kg bw SMN. ALT, AST and total & direct bilirubin were investigated in serum. SOD and CAT were investigated in hepatic tissues. Also, the gene expression of SOD, CAT, p53, and Cox-2 was determined in hepatic tissues. After statistical analysis our results referred to the following: ~ Serum ALT & AST activities and total & direct Bilirubin levels: DENA group revealed significant increase in the level of total& direct bilirubin and in the activities of ALT & AST but silymarin -treated rats revealed improvement in these liver functions. ~ Superoxide dismutase and Catalase activities in Hepatic homogenate: |