الفهرس 
Introduction of LiteratureOnly 14 pages are availabe for public view
 |  | from | 114 |  |  |
| | | from | 114 | | |
Abstract
This study was conducted to evaluate beta catenin expression in oral premalignant lesions and oral squamous cell carcinoma and to investigate the. possible correlation that might exist between beta catenin expression and different grades of epithelial dysplasia and oral squamous cell carcinoma.
Fifteen cases of OED were selected, and classified according to severity of dysplasia into 6mild, 6 moderate and 3 severe cases, and twenty cases of oral see were selected, and classified according to degree of differentiation into 7 well differentiated, 9 moderately differentiated and 4 poorly differentiat<yd. Using peroxidaseeantiperoxidase method for immunohistochemical examination, each specimen was stained with beta catenin antibody.
For result interpretation, a quantitative assessment of beta catenin antibody expression was carried out using the image analysis software (Image J, 1.41a, NIH, USA).
Results of the present study revealed that all cases of premalignant lesions were immuno-positive, and all cases revealed nuclear expression of ~-catenjn.The immunohistochemical results of oral squamous cell carcinoma revealed that all cases are immuno-positive, while the localization of beta catenin was different, as it seen in 17 cases (85%) in cytoplasm, 2 cases (10%) showed cyto-membranous expression, and one case (5%) showed nuclear expression.
______ ----I( 74 )-------
Summary
Statistical analysis of the present study regarding p-catenin expression in oral epithelial dysplasia revealed a significant statistical difference between mild, moderate, and severe cases of oral epithelial dysplasia (p=O.Ol), where the mean area fraction of immunopositivity for mild, moderate and severe cases were (15.9804, 5.5249 and 2.9174) respectively. These results mean that the expression of p-catenin decreased when severity of epithelial dysplasia increased and adhesion between cells decreased.
Statistical analysis of the present study regarding p-catenin expression in different grades of oral squamous cell carcinoma revealed insignificant statistical difference between well differentiated, moderately differentiated, and poorly differentiated cases of oral see (p=0.292). These data suggested that p-catenin is not a reliable marker for differentiating different grades of see.
Statistical analysis of this study revealed that beta catenin expression showed a strong negative inverse correlation in different grades of OED cases (r = - 0.817). This correlation was proven to be statistically significant (p = 0.0001). This means that when severity of OED increased the expression of beta catenin decreased. Our results suggest that p-catenin function in cell-cell adhesion and translocation in the nucleus are related to proliferation of OED. However statistical analysis regarding p-catenin expression in different grades of oral see revealed weak negative inverse correlation between p-catenin expression and severity of see (r = - 0.411). But this correlation was statistically insignificant