الفهرس | Only 14 pages are availabe for public view |
Abstract Studies were made on 12 different bacterial isolates.Bacillus subtilis E was found to be the most potent calcium lactobionate producer. The conditions of cultivation as well as the conditions of calcium lactobionate production byB. subtilis Ewere investigated. The highest calcium lactobionate yield (90%) was obtained using production medium composed of (g/100ml) :lactose ,5; KH2PO4,0.06 ; MgSO4.7H2O, 0.025; corn steep liquor ,0.75 ml ; 1 DROP of soybean oil ; 1.5ml of 20% urea and CaCO3, 2.5 gm added during inoculation. The medium (100ml) was inoculated with 3ml inoculum and incubated at 30˚C for 7 days under shaking condition 200 r.p.m. Calcium alginate, agar-agar and chitosan were used as natural and synthetic carriers for immobilization of B. subtilis E. The production of calcium lactobionate is performed efficiently by adsorption of cells on chitosan sheets. Key Words: Microbial lactose conversion, calcium lactobionate, Physiological factorsand immobilization. |