الفهرس 
Introduction and Aim of The WorkOnly 14 pages are availabe for public view
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Abstract
This study aimed to explain some effects that arise from human lead daily exposure at polluted sites as well as male mice treated orally by lead acetate (2000 and 4000 ppm) in drinking water.This experiment was established on both human volunteers and experimental adult male mice as follow:
1-Human subjects
Selected 30 male volunteers after having a written informed consent, as research ethics establishing, they were divided as follow:
Group (1) as control: they are15 healthy adult human subjects from nearly no or less lead pollutant exposed area as possible.
Group (2) lead exposed: 15 male exposed daily to lead from their work in bus stations and battery factories, painting, or working in crowded situations by cars.
We get approximately 10 cm of blood from each volunteer for laboratory investigations.
2-Animals:
30 adult male mice 25 ± 2g were divided into 3 groups:
Group (1): control healthy male mice. (n=10)
Group (2): lead acetate (2000 ppm) treated male mice orally through drinking water for 10 weeks. (n=10)
Group (3): lead acetate (4000 ppm) treated male mice orally through drinking water for 10 weeks. (n=10).
Body weights were recorded weekly through the experimentation period, at the end of experiment, mice were sacrificed where a part of blood left to clot, then centrifuged to get sera and another part collected on heparin as anticoagulant for red and white blood cells count and determination for Hb content and Ht %.
Mice brains quickly separated then homogenized in bidistilled water for antioxidants and neurotransmitters determination.
Estimated parameters:
Lipid peroxidation product (MDA) were determined , reduced glutathione (GSH) level , and activity of super oxide dismutase (SOD), also some brain neurotransmitters such as serotonin and dopamine, as well as, some hormones such as thyroxin, triiodothyronine, cortisol and testosterone. In addition, some metals concentration such as lead , iron and zinc, were determined in blood or serum.
Obtained results, are:
Lead exposure caused decrease in body weight gain, disturbance in red and white blood cells count, decrease in hemoglobin content and hematocrit percent which lead to anemia. Significntly decrease in SOD activity and GSH content, and increase level of MDA. Also. Significant decrease in thyroid hormones T3 and T4, as well as testosterone concentration parallel with increasing cortisol level.Increase in serotonin level, and on other hand decrease in dopamine level.Decrease in serum Zn and Fe levels, in concomitant with increased Pb concentration in red blood cells.
We can conclude from this study that, lead exposure may cause disturbance in the body balance of Zn and Fe and physiological deficits for nervous system functions and oxidative stress. In concomitant with decrease in thyroid hormones testosterone accompanied by increase in cortisol concentration in both human and experimentally Pb exposed mice which pointed to the danger on the health of both human and animals so, it is recommended that we must be far away -as possible- from this pollutant area to safe our health.