الفهرس 
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Abstract
Neutrophils are the most abundant type of phagocytes.The main products of the neutrophil respiratory burst are strong oxidizing agents including hydrogen peroxide, free oxygen radicals and hypochlorite.The vital importance of NADPH oxidase. Meanwhile, PKC plays a central role in activation of NADPH oxidase enzyme leading to the production of ROS during neutrophil oxidative burst. However, its exact role needed to be deeply investigated at the molecular levels to conform with its published biochemical role in the activation of the oxidative burst. a series of experiments done to answer the prepared objectives.
Results can be summarized as follow:
The viability of neutrophils were not affected at all by tricine and salicylic acid while CuCl2 and Cu-complex reduced their viability by 26% and 12% respectively at the used concentration of 1000 μΜ.
Only three DNA sequences which encode isoforms: PKCγ, PKCε, PKC λ were detected in the Egyptian buffalo᾽s neutrophils.
The OZ and zinc chloride stimulated the respiratory burst in concentration dependent manners which were proportional to the mRNA levels of the three PKC isoforms.
whereas, the neutrophils that incubated with salicylic acid and tricine-copper complex then stimulated with OZ inhibited the oxidative burst and hence ROS production.
However, their effects at the molecular levels; the salicylic acid increased the transcriptional and expression levels of PKC whereas, copper complex showed an initial increase in transcriptional level then a decrease with longer incubation period.
In conclusion, the current set of data resulted in the discovery of the existance of lower number of PKC isoforms in Egyptian buffalo᾽s neutrophils than what is widely reported in the literature. Also, the locally prepared tricine-copper complex proved to be an extremely potent inhibitor at all concentrations used, depsite the known stimulatory effect of Cu ions at lower concentration for the oxidative burst.