الفهرس 
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Abstract
In the present NDV inactivated vaccine was prepared and tetanus toxoid was added as immunopotentiator five groups of SPF chicks were vaccinated with the prepared lyophilized inactivated vaccine by (I/N, I/O, S/C) routes and HB1 and inactivated paraffin oil NDV vaccine. Results of testing cell mediated immunity and humoral immunity revealed that the prepared inactivated lyophilized vaccine induced significant high lymphocyte and macrophage cells compared to the effect of HB1 and paraffin oil inactivated vaccine at P value ≤0.05. In conclusion, the study highlight the add value of using inactivated lyophilized NDV vaccine as priming and booster with protection percent 80% with I/O and I/N route and 100% with S/C route after two doses with two weeks interval, using the inactivated lyophilized NDV vaccine as primary vaccination followed by inactivated paraffin oil NDV vaccine as a booster instead of live attenuated vaccine provides robust immunity with longer duration till 22 weeks.