Search In this Thesis
   Search In this Thesis  
العنوان
Genotypic Methods for Identification of Candida Species Isolated from Human and Animal Infections /
المؤلف
Afify, Marwa Naeem Hegazy.
هيئة الاعداد
باحث / Marwa Naeem Hegazy Afify
مشرف / Mohamed Taha Mahmoud El Sayed
مشرف / Mohamed Taha Mahmoud El Sayed
مشرف / Mohamed Taha Mahmoud El Sayed
الموضوع
Candida. Veterinary Mycology.
تاريخ النشر
2012.
عدد الصفحات
133 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2012
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Department of Bacteriology, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

from 141

from 141

Abstract

The genus Candida exists as commensals of mucosal membranes in most healthy individuals and other warm-blooded animals, where they grow without causing any damage. In the present study, a total of 192 samples were collected from El- Sharkia, Ismailia, and Dakahlia Governorates, recovered from cattle, chicken and human as a source of various biological specimens, which included skin, vaginal, oral, rectal, nasal swabs and milk, crop samples, and nail scraping. After primary examination on SDA and Gram’s stain, the examined samples revealed macroscopically growth of white to creamy colored yeast colonies varied between smooth, glistening or dry, or wrinkled and dull colony texture. As well as microscopically revealed Gram positive spherical to oval yeast cells, irregular in size, some showing budding yeast cells. The isolation of 95 out of 192 yeast isolates was revealed in a total percentage of (49.5%), where the percentage of yeasts isolated was higher in chicken (75%) than that isolated from different human samples (55.5 %) and cattle (43.3 %). The percentage of positive samples obtained from cattle for yeasts was highest from rectal swabs (76.2%), followed by oral swabs (61.5%), while, the positive yeast samples recovered from human with high percentage were oral swabs (65%), then vaginal swabs (35%). In the present work , out of 95 yeast isolates examined on rice agar with 1 % Tween 80 at 25°C for 3 days. Eighty five yeast isolates showed various criteria of Candida species.