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Abstract 520. التبصرة الخاصة بالت... ## a) A total of 250 st rains of ~. aeruginosa were isolated different patients, stools of healthy individuals as as the soil at different localities in Egypt. Out of • strains, 91 strains were isolated from f’-1isr Spinning Weaving Co. hospital (54 from urine specimens, 10 from burn cases and osteomyelitis, 6 from corneal 98 cases, 5 from otitis media and 2 strains from each ~st1c fibrosis, septic wounds and stool). 84 strains isolated from Mansoura University hospital (29 from stool, 16 from septic wounds, and 6 from each of septic burn cases damage. 21 strains were isolated from General Sa~annud (20 from. urine specimens and one septic wound case). 18 strains were i501aafrom Burn Institute (Cairo). 12 strains were isolated om urinary tract infection cases in f’-1ebarra hospital Kobra) and 8 strains were isolated from General of Mehalla Kobra (3 from both urine specimens septic burn cases and 2 from chronic otitis media Finally, 16 strains’ were isolated from soil. isolated strains were examined using different para- find the most suitable system for proper identtand determination of source of infection. studies between the isolated strains were the phage typing, pyocin typing (active and 8sive), antibiotic resistance, production of pyocyanin -174- production including protease, lecit~inase and n addition to the aminoglycoside-inactivating nd out as many differences, similarities or 1ps as one can trace between the isolated strains, data processing was carried out using a computer e Apple 2,48 k. cling antibiotic resistance, it was found that e isolated strains were resistant to streptomyyprobably this may be due to the extensive use tibiotic in Egyptian hospitals, on the other acin and polymyxin-B resistance was the least ’9 only 2% of the isolated strains. There was cant correlation between antibiotic resistance 1d locality, except with strains having the pattern streptomycin/kanamycin/colimycin that Lsolated from Mansoura University hospital. 19S may indicate the role played by general 3nd common wards of surgery in the spread of nosocomial organism with definite antibiotic pattern. There was a correlation between resistance and clinical sources of infection, ;bserved that the burn, corneal daw~ge and ld isolates were more resistant to kanamycin ~her clinical isolates (78.4%, 79% and 58%. |