الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 230 |  |  |
| | | from | 230 | | |
Abstract
Recurrent pregnancy loss is a common problem for patients and physicians alike. One of the most attributed causes of this problem is recurrent uterine infection by Mycoplasma hominis or Ureaplasma urealyticum. The present study was undertaken to evaluate the role of genital mycoplasma in early pregnancy wastage.
• The first group (50 pregnant women in the first trimester with history of spontaneous abortion) 50 subjects: 35 cervical swabs, 15 vaginal swabs, and 50 blood samples were taken.
• The second group (50 pregnant women in the first trimester without history of spontaneous abortion) 30 cervical swabs, 20 vaginal swabs, and 50 blood samples were taken. This group serving as a control for the first group.
• The third group (50 recently aborted women in the first trimester with history of habitual abortion) 40 cervical swabs, 10 vaginal swabs, and 50 blood samples were taken.
• Tile fourth group (50 recently aborted women in the first trimester without history of habitual abortion) 30 cervical swabs, 20 vaginal swabs, and 50 blood samples were taken .
• rile fiftll group (100 married nulliparous womwn with history of primary infertility) 60 cervical swabs, 40 vaginal swabs, and 100 blood samples. This group acting as a general control.
Genital mycoplasmas were isolated and identified using different morphological, biochemical and serological methods.
For Ureaplasma urealyticum, 6 types of broth media [U.9~C, 10C, B.T.B., Shepard, Lee and Foy media] and 5 types of agar media [ASK, A8, G .M, Stand.H, and T.media] for· primary isolation and identification. The best media for growth and isolation was A8 and 10C media for agar and broth.
Four types of media at pH 7.6 were used for recovery of other genital mycoplasmas [SP4, S. mediwll, Sacy medium, and modified Hayflick mediwTI]. Medium Sacy was the ideal medium for the isolation of Mycoplasma homil1is and Mycoplasma genitaliwll.
On solid media most mycoplasma form very small colonies (50-600 I- .. UTI. in diameter) and have a characterized ”fried egg” appearance.
Ureaplasma urealyticwn produced very small tiny colonies (15-30 J-!m.) in diameter.
We applied the following tests for identification of Ureaplasma urealyticum:
1- Urease colour test (A8 mediwn) which is a special agar medium for isolation and specific identification of U.u. in primary cultures of clinical material. The colonies of V.u. appeared brown black in colour.
2- Sensitivity to 1-2000 tho acetate.
3- Sensitivity to 200 J-!gm/liter lincomycin.
4- Four serological tests were used (agar· gel precipitation, growth inhibition, metabolic inhibition test, and Dot Eliza test). The most sensitive and specific serological test was growth inhibition test.
5- The incidence of V.u. among the five groups examined was 52% in the first group, 30% in the second group, 66% in the third group, 28% in the fourth group and 12% in the fifth group.
For identification of Mycoplasma 110m on is we used the following: 1- Erythromycin sensitivity test, Mycoplasma hominis was the only genital mycoplasma which is resistant to 100 J-!gm/liter erythromycin. 2- M.h. produced large fried egg colonies.
3- Mh. was arginine hydrolysis +ve.