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Abstract Poultry production plays an important role as good and cheap source of animal proteins in Egypt. The extensive and misuse of antibiotics for control of disease and growth promotion resulted in a problem of drug residues. Prevention of residues in meat and poultry is a top of priority of the public health authorities. Recently, residues in meat and poultry represent a major problem facing the hygienists. Now the public health authorities in different countries had established’ regulations of the Veterinary use of the drugs in food of animals and poultry origin. So, the present study was performed to determine the presence of antibiotic residues in locally and imported frozen chicken meat and also the detection of effect of cooking procedures (boiling, frying and roasting) on positive samples that contain antibiotic residues.so, the following experiment were performed. I)Qualitative detection of antibiotic residues in random market froze chicken meat samples using the microbiological assay technique: A total of 120 fozen chicken samples were collected from chicken shops and supermarkets at Dakhahlia Governorate, Egypt. All samples were examined for presence of antibiotic residues using microbiological inhibition test. The freshly prepared Bacillus’ subtilis plates was used and incubated at 30°C for 24 hours. The results revealed that the incidence of antibiotic residues as follow no residues was detected in imported frozen chicken meat but the residues concentrated in local frozen chicken meat as follow |