الفهرس | Only 14 pages are availabe for public view |
Abstract A crossbreeding experiment was carried out in Sakha Animal Production Station, belongs to the Animal Production Research Institute, Agricultural Research center, and the molecular studies were carried out at Biotechnology Research Center (BRC), Suez Canal University. This study has used three different genetic techniques to improve some economic traits in crossbreed rabbits established by crossing between Gabali and V- line. An experimental population was specifically generated with the aim to detected QTL for a number of different economically important traits including body weights at 4, 6, 8 wks of age. The population is based on a so-called full sib design based on two generation. The total genome scan was performed on ten full sib families consisting of 61 GO and 448 G 1 animals which were typed with 20 micro satellite markers covering 2 chromosomes and a full sib across family regression interval mapping. approach. A significance thresholds of 5%, 10% was used and calculated by permutation test to identify if there is QTL affecting for body weightsat 4, 6, 8 wks of age or not. The number of micro satellite markers successfully tested on QCU1 and QCU13 were 10 and 10, respectively. 3 markers out of these 20 markers appeared to be uninformative markers leaving 17 markers to be used for the present study.To better understand the relationship between Gabali breed, V -line and their crosses in This study is to evaluate genetic diversity by using •blood protein analysis by use of electrophoregrams of protein separated in SDS polyacrylamid gel. The electrophoresis in this type of gels allows obtaining up to 64 protein bands with the average of 16 bands for each lane of the gel, the separated gel picture analyzed on a Gel Documentation System. A data matrix was constructed by using Gel Quest software and these matrices were analyzed by using Cluster Vis software packages to determine Genetic similarities and distances among the rabbits population studied. |