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Abstract Fifty male albino rats were used in this study. They were classified into five equal groups, the first one was used as a control. The animals of the second group were given methotrexate (2.5 mg/kg /d) orally for 3 days. The rats of the third group were given methotrexate (as in Group II) and ascorbic acid (150 mg/kg/d) for 2 weeks before methotrexate treatment. The rats of the fourth group were given methotrexate (as in Group II) and curcumin (100 mg/kg/d). The rats of the fifth group were given methotrexate (as in Group II), ascorbic acid (as in Group III) and curcumin (as in Group IV). At the exact time of the experiment, the rats from each group were sacrified and parts of their jujenum were dissected out, carefully removed and divided into small slices; the first was fixed in Bouin’s fixative and stained with hematoxylin and eosin, mallory stain, periodic acid schiffs reaction (PAS) and Grimelius’ silver stain for argyrophilic granules. from the second slice, fresh frozen cryocut sections were prepared and stained for alkaline phosphatase activity. The third slice was processed for ultrastructural study. Quantitative morphometric studies were done to determine length of the jejunal villi and the number of goblet cells in the upper (tip) and lower (base) halves of the jejunal villi for each group. |