الفهرس 
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Abstract
In the present study, The present study determined the extent of production of some virulence factors and antimicrobial resistance of P. aeruginosa isolated from different clinical sources in Mansoura University Hospitals. A total of 98 isolates of P. aeruginosa were isolated from specimens collected. The susceptibility to 10 antimicrobials was determined and analyzed among isolates. The isolates were investigated for their possession of virulence factors including pyocyanin production, hemolysin production, proteases (total proteases and staphylolytic activity of elastase A), lipase production and biofilm formation. In addition, the effect of repeated exposure of P. aeruginosa to sub-inhibitory concentrations of three of commonly used SAAs (tween 80, SDS and cetrimide) on the susceptibility to antibiotics and the extent of some virulence factors production was studied. Also, the effect of exposure of the tested isolates to high concentrations of SDS and cetrimide for a short time (1 hr) was studied. Five bacterial genes that encode virulence determinants (toxA, aprA, lasB, exoU and exoS) were characterized on both chromosomal and plasmid DNA using PCR technique.