الفهرس 
review of literatureOnly 14 pages are availabe for public view
 |  | from | 236 |  |  |
| | | from | 236 | | |
Abstract
Both flow cytometry and static image analysis have been used to
determine DNA content (ploidy) of prostate lesions. Quantitative DNA
variables were evaluated in prostatic core biopsies from patients with
various prostatic lesions. An increase of DNA content was observed from
normal to cancerous tissues. Objective: The goal is to evaluate the role of
the image analyser system in differentiation between benign, precancerous,
and malignant prostatic lesions. Also, a correlation will be done between the
morphometric and DNA ploidy data.
Study design: The present study included 60 prostatic core biopsies
consisting of 20 cases of benign prostatic hyperplasia (BPH), 20 cases of
prostatic intraepithelial neoplasia (PIN), 20 cases of prostatic carcinoma and
5 cases as a control. DNA content analysis of sections stained with blue
Feulgen stain and nuclear morphometrical analysis of the haematoxylin and
eosin stained sections were carried by using the Leica Qwin 500 image
analyzer. Result: All benign lesions included in this study were diploid (2C)
and all the malignant lesions were aneuploid (>4C) except one case which
was diploid. The diploid DNA value was significantly higher in the normal
group than in all other groups (p=0.000); and lower in the carcinoma group
than in all other studied groups (p=0.000). Also the diploid value was
significantly lower in PIN group than in BPH groups (p=0.000). The
proliferation index (S-phase) value was significantly higher in all groups
when compared to normal (p<0.008). The aneuploid value was significantly
higher in carcinoma group than in all other studied groups (p=0.000). The
normal group did not show any aneuploid values. The mean nuclear area in
carcinoma group was significantly higher than that of normal, BPH and PIN
groups (P=0.000).Conclusions: The DNA ploidy analysis helps to
differentiate between normal, BPH, PIN and malignant groups. Distinction
4. ABSTRACT
9
between PIN and cancer could be approached on more objective criteria by
DNA and nuclear morphometrical analysis.
Key words: morphometry, nuclear area, BPH, PIN and prostatic carcinoma.