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Abstract Propolis is a resinous material collected by bees from bud and exudates of the plants which transferred in the presence of bee enzyme into other complex substance composed of 30% wax, 50% resin and vegetable balsam, 10% essential and aromatic oil, 5% pollen and other substance. Bees use it to seal holes in their honey combs, smooth out internal wall as well as to cover carcasses which died inside the hive in order to avoid their decomposition. Propolis has protective and therapeutic effect against many diseases, bacteria, virus, fungus and turnors for thus; this study was conducted to evaluate the effect of propolis on albino rat infected with Trypanosoma lewisi and also its effect on pigeon squabs infected with Trichomonas gallinae. Isolation of strain of Trypanosoma lewisi was done from chosen infected wild rats captured by traps from field and banks of river Nile canals of Mini a El-Kammah at Sharkia province and maintained in 50 albino rats (each of 150 gm body weight) by intra-peritoneal inoculation of heparinized blood containing Trypanosoma lewisi collected from the infected wild rats. Strain of Trichomonas gallinae was done from infected squabs collected from lofts and morbets of Minia El-Kammah at Sharkia province and maintained in squabs by oral route inoculation and propagated in culture of glucose broth serum medium and left until used. Propolis collected by scrapping off the frames of beehives located at Sharkia province, 3% water extract of pro po lis was prepared. |