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Abstract The aim of this study was to evaluate in vitro the effectiveness of two different apex locators (Root ZX II and Csmart I) in detecting simulated horizontal and vertical root fractures of different thickness and different location. A total of 60 extracted teeth were inspected for the absence of cracks, perforations, fractures or any defects. Teeth were decoronated at the cementoenamel junction and adjusted to 15 mm in length. Samples were classified into two main groups, one for simulated HRFs (40 teeth) and the other for the VRFs (20 teeth). The first group with HRFs was subdivided into two subgroups according to the location of fracture, coronal (5 mm away from the coronal surface) and apical (10 mm away from the coronal surface). Then each subgroup was further subdivided into two subdivisions according to the size of fracture, 0.15 mm and 0.20 mm (10 teeth each). The second group was subdivided into two subgroups according to the size of fracture, 0.15 mm and 0.20 mm (10 teeth each). Five teeth were used as control without any further preparation. Each root was fixed in a copper mould using acrylic resin. Moulds were then held against the disk to simulate HRF and VRFs using copious amount of water coolant until the canal got exposed. The simulated VRF was started with the disk touching the root surface at 7.5 mm from the coronal surface. To verify canal exposure, sterile saline was injected in each canal and saline leakage from the simulated fracture site was examined visually with the help of cotton pellets. To confirm the actual vertical fracture level for group II with VRFs, small K-file #8 or #10 was then introduced through the cut perpendicular to the long axis of the root. A digital radiograph was then taken and the software used to calculate the actual fracture level. Resinous cast (model) was used to fix the teeth. Alginate impression material was mixed and poured into it and roots were immersed in it before setting. One lip clip was immersed in the alginate before setting and the file holder attached to K-file# 15. This K-file# 15 attached to the file holder was then introduced into each root canal until the meter reaches the mark ”apex” on the screen of each EAL and the beeping sound heard; the stopper was set on the flat surface of the root (reference level) and the distance measured. Measurements were obtained immediately after setting of the alginate impression material. All samples were measured first using Root ZX II apex locator and then measured by using the Csmart I apex locator. A percentage was calculated for each reading using the following equation: (Apex locator reading x 100)/Actual fracture level. The difference in measurement was calculated as the absolute difference between the apex locator reading and the actual fracture level measurement. Data were collected and statistically analyzed. Results: Root ZX II readings were shorter than the actual fracture level with a statistically significant difference for the simulated 0.20 mm apical HRF (I2b). No statistically significant difference was shown between the other three subdivisions with simulated HRF and the actual fracture level using Root ZX II. No statistically significant difference was shown between different fracture thickness, 0.15 mm and 0.20 mm (subdivisions I1a and I1b) and (subdivisions I2a and I2b) coronally or apically using Root ZX II. There was a statistically significant difference in detection of 0.15 mm and 0.20 mm HRFs coronally and apically (subdivisions I1a and I2a; I1b and I2b) using Root ZX II. There was no statistically significant difference between the mean of the C-Smart I readings and the actual fracture level for all subdivisions of group I with simulated HRFs (I1a, I1b, I2a, I2b). Also no statistically significant difference was shown in detection of simulated HRFs using the C-Smart I for different simulated fracture thickness within the same level (subdivisions I1a versus I1b coronally and subdivisions I2a versus I2b apically). There was no statistically significant difference shown in detection of simulated HRFs using the C-Smart I for the same thickness fractures at different levels (subdivisions I1a versus I2a 0.15 mm and subdivisions I1b versus I2b 0.20 mm). There was no statistically significant difference between the mean of the Root ZX II reading and that of the C-Smart I for subdivisions I1a, I1b, I2a and I2b with simulated HRFs. There was no statistically significant difference between the mean reading and the actual fracture level for both subgroups with simulated VRFs (Iia and Iib) using both apex locators. No statistically significant difference was shown in detection of simulated VRFs of different thickness (subgroups Iia and Iib) using both apex locators. There was no statistically significant difference between the mean of the Root ZX II reading and that of the C-Smart I for both subgroup with simulated VRFs. There was a statistically significant difference between the mean percentage value of accuracy of the Root ZX II readings for group I (HRFs) and group II (VRFs). There was a statistically significant difference between the mean percentage value of accuracy of the C-Smart I readings for group I (HRFs) and group II (VRFs). |