الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 134 |  |  |
| | | from | 134 | | |
Abstract
This study was carried out at Tissue Culture Laboratory in the Department of
Horticulture, Faculty of Agriculture, Suez Canal University at Ismailia throughout the
period of 2008-2010. The aim of this study was to set a protocol for the in vitro culture of
Strelitzia reginae through controlling of both seeds dormancy and phenolic compounds
secretion phenomena, and also increasing the multiplication rate through wounding of the
meristem region which reduce or eliminate apical dominance and subsequently simulate
the growth of axillary buds.
Results showed that, culturing excised embryos during germination stage on solidified
MS medium supplemented with 2g/l Activated charcoal (AC) effectively reduces
oxidative browning and also the brown exudates diffusing into the medium which have
detrimental inhibitory effect on explants development with a germination percentage of
12.5 %.
For dormancy breaking media, gradual elevated concentrations of GA3 alone, the best
GA3 concentration in combination with different concentrations of BA and the best GA3,
BA concentrations with different sucrose concentrations were used. The highest
germination percentage (70 %) was recorded when 0.5mg/l BA+0.8 mg/l GA3 + 30g/l
sucrose were added to the MS medium.
The best results for proliferation stage were achieved through culturing S. reginae
meristematic region (1 cm ± 0.2 cm) after meristem wounding on MS medium
supplemented with 8 mg/l BA in addition to 0.5 mg/l NAA with pH adjustment to 4.5.
Overall, the obtained results assume that using GA3 in addition to BA breaks S. reginae
seeds dormancy and making several incisions to apices plus using high BA concentrations
jointed with low NAA concentrations is successfully simulate proliferation.