الفهرس 
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Abstract
The present work was designed to study the possible role of . inflammation induced by E. coli in the urinary bladder of rat, potective role of curcuma, in addition to the early detection of (as proliferative index) and caspase-3 (apoptotic modulator) in carcinogenesis. The present study was performed on one and fifty adult male albino rats. They were divided into five
groups; a) Normal control group, b) Dibutyl amine (DBA) and nitrate (nitrosamine precursors) -treated group, c) E. coli group, d) Dibutyl amine and sodium nitrate (nitrosamine ) - treated group plus E. coli, and e) Dibutyl amine and nitrate (nitrosamine precursors) - treated group plus E. coli curcwna rhizomes, each -included 30 rats. Rats were sacrificed each 3, 6 and 9 months of daily oral administration of dibutyl (DBA) combined sodium nitrate (nitrosamine precursors) in
. water, curcuma combined in grinding basic diet and bladder with E. coli,. The excised bladder were dissected, processed
ltained with H&E and anti-Ki67 immunohistochemical stains. followed by Elisa for caspse-3 and statistical analysis. Ki67 aamined using immunohistochemical LSAB method which is the sensitive method. Caspase- 3 was examined using ELISA Molecular alterations analysis was done using the SDS¬lamide gel for protein electrophoresis. Survival rate and body changes were recorded during the period of treatment.