الفهرس 
Cover PageOnly 14 pages are availabe for public view
 |  | from | 179 |  |  |
| | | from | 179 | | |
Abstract
Lake Qarun is a big natural reservoir for agricultural/municipal drainage coming from
different localities of Fayoum province. Thus, investigating the possible presence of fish
pathogenic bacteria is a core goal while studying the impact of water quality measures on
the lakes ecosystem including the most common inland fish species, Solea aegyptiaca.
Randomly one hundred Solea fish were collected from Shakshouk area, Lake Qarun,
Fayoum Province, Egypt through different seasons of the year. Investigation revealed
that most commonly isolated bacteria were V.alginolytcus, V.cholrea, V.fluvialis,
V.vulnificus, Photobacterium damselae subspp. damselae, Ps.anguilliseptica,
Tenacibaculum maritimum, Staphylococcus warneri and Pasteurella multocida. Total
incidence of bacterial infection was 76 %, with seasonal incidence of 68% in Spring,
76%in Summer, 80% in Autumn and 80% in Winter. Polymerase Chain Reaction (PCR)
was used for identification of the previously isolated Pasteurella multocida using the
species specific primers of (pm1132) gene and the amplified product consisted of 489Bp
fragment.Clinically examined fish exhibited dark discolouration, erosions and
heamorrhages of body parts.The target organs for isolation were kidney, liver, spleen
andgills. Histopathologically congestion with fattychange and focal heamorrhage in
Liver.Aggregation of melanomacrophage cells all over the renal parenchyma in
kidneyandNecrosis of gill lamellae, hyperplasia and fusion of gill lamellae and seveer
congestion in blood vessels of Gills. Analysis of the retrieved clinicopathological,
bacteriological as well as water quality measures has drived us to conclude that the
ongoing deterioration of the lake’s water imposed by enviromental pollution is the main
trigger behind such bacterial invasion in Solea fish.