الفهرس 
coverOnly 14 pages are availabe for public view
 |  | from | 128 |  |  |
| | | from | 128 | | |
Abstract
The study is to assess the mode of action of some solvents as
a protecting agent during human embryos freezing
(cryopreservation, vitrification). Survival rate of human embryos
had been assessed after vitrification process using two
different freezing (vitrification) media with two different
solvents; Dimethylsulfoxide (DMSO) based medium and 1, 2
Propandiol (PROH) based medium. This two solvents act as
cryoprotective additives (CPA) that provide a protective effect
for human embryos vitrification. Day2 or day3 divided human
embryos were exposed to DMSO or PROH based medium
before plunging into Liquid Nitrogen (LN2) for long term
storage. 212 embryos in vitrification process were split into two
groups. Group A (114 embryos) were vitrified using sequential
vitrification medium based on DMSO and Group B (98
embryos) were vitrified using sequential vitrification medium
based on PROH. In the Warming procedure (Thawing)
embryos of the group A and B were thawed respectively.
Thawed embryos were transferred to physiological media
designed for cleavage stage embryos and kept in the CO2
incubator for 2 to 8 hours. The survival rate of embryos was
evaluated. After vitrification/thawing procedure, the survival
rates of group A (DMSO) and group B (PROH) were 85
survived embryos of 114 (74.6%) and 52 survived embryos of
Abstract
vi
98 (52%) respectively (P.value<0.05). DMSO based medium
used for vitrification/thaw process of day 2 or day 3 divided
embryos demonstrated a significant higher embryo survival
rate than that of PROH based vitrification medium. Results had
been analyzed chemically to illustrate the mode of action of
DMSO and POH and to explain the superiority of DMSO over
POH.