الفهرس | Only 14 pages are availabe for public view |
Abstract This work aimed to carry out a molecular and phenotypic based characterization of APEC isolated from different f0TI11S of colibacillosis in Egyptian poultry flocks and compare these isolates with AFEC and environmental E. coli. Further analysis characterized APEC associated with colisepticemia cases the most prevalent form of colibacillosis. In addition the identification of the immunogenic epitopes that could be used as candidates for APEC vaccine development. Moreover this study utilized the molecular cloning as a tool for the production of a candidate recombinant vaccine against APEC infection. Experiment 1: For characterization APEC isolated from different forms of colibacillosis in Egyptian poultry flocks and compared these isolates with AFEC and environmental E. coli. In addition further analysis characterized APEC associated with colisepticemia cases. In this experiment, 219 E. coli isolates from 84 poultry flocks located in four provinces (Sharkia, Dakahlia, Gharbiyah and Ismailia) including 153 APEC, 30 avian fecal E. coli (AFEC) and 36 environmental E. coli, were subjected to phylogenetic grouping and virulence genotyping. Additionally, 50 of these isolates (30 APEC from colisepticemia and 20 AFEC) were subjected to a more extensive characterization, which included serogrouping, antimicrobial susceptibility analysis, screening for seven intestinal E. coli virulence genes (stxl, stx2, eae, espP, KatP, hlyA and fliCh7), multilocus. |