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العنوان
Studies on spectroscopy as a method for identification of yeasts and dermatophytes isolated from human and animals with special reference to antifungal sensitivity test for identified isolates /
المؤلف
Tartor, Yasmine Hasanine Ibrahim.
هيئة الاعداد
باحث / Yasmine Hasanine Ibrahim Tartor
مشرف / Mohamed Taha Mahmoud
مشرف / Mohamed N abil Hassan
مشرف / Mycology and Immunology
الموضوع
Dermatophytes - pathogenicity. Zoonoses diseases. Veterinary Mycology.
تاريخ النشر
2013.
عدد الصفحات
144 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2013
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Department of Bacteriology, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

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Abstract

dermatophytes including phenotypic and PCR methods. Recently, FT - IR spectroscopy is increasingly being used for identification of yeasts and dennatophytes. This study aimed to investigate the capability of this technique to identify and discriminate between the most frequent yeast and dermatophytes species isolated from human and animals. To that end, yeast and dermatophytes isolates were identified by phenotypic method, RFLP, RAPD PCR and FTIR spectroscopy analysis. A total of 166 isolates (128 yeasts and 38 dermatophytes) were isolated from 266 samples represented in: 75samples from human cases (20 vaginal, 20 oral swabs and 35 skin scrapings , hair and nail scrapings), chronic diseased cows (131 sample including 21 rectal, 20 vaginal, 26 oral, 19 nasal, 20 ear swabs and 20 mastitic milk in addition to 5 skin scrapings from cattle ringworm), chicken crop mycosis (20 swabs) and five cases of dog dermatophytosis. In addition to, thirty five soil samples including 25 soil contaminated with pigeon droppings for isolation of Cneoformans and 10 soil samples for isolation of geophilic dennatophytes. The present work revealed that, while genotypic method and FfIR could identify yeast and dermatophytes into levels of genera, species and even to varieties. Phenotypic methods not enough to identify most yeast species and dermatophyte varieties. Phenotypic identification of yeasts isolates revealed that, the most Candida spp. isolated from human oral and vaginal candidosis was Celbicens (30%) followed by C tropicelis (5%) then Cperspsilosis and Cglabrata (2.5%) for each.