الفهرس 
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Abstract
irology.This work was carried out for molecular comparison between FMDV field strains causing recent outbreaks in Egypt
and the used vaccine strains. 252 epithelial samples and 13 8
oesophageal pharyngeal fluids were collected from 9 different
Egyptian governorates during the period of2009 to 2012.
The result obtained from this study can be summarized as followings: .
1. Isolation and identification of the FMDV current field strains
by tissue culture inoculation, baby mice infection and by R T-
PCR using universal primer.
The collected samples were isolated in BHK cell line for
three successive passages and also by baby mice inoculation
observed for seven days, all the suspected samples are
positive. The positive samples then retested by RT-PCR for
general detection of FMDV using universal primer, all
samples are positive.
Typing of the isolated FMDV strains by CFT using specific
guinea pig antisera to the 146S antigen of each of the seven
serotypes of FMDV, ELISA using specific rabbit antiserum as
a trapping antibody, and specific guinea pig antisera as the
detecting antibody, and RT-PCR using RNA extraction by
GeneJET™ RNA Purification Kit (Fermentas, EU), RT-PCR
by Maxime” RT-PCR PreMix Kit (iNtRON, Koria) and five