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Abstract Fifteen fresh soft cheese samples (Kareish and Damietta) were collected from shopkeepers, retail stores and rural markets. A survey was conducted to determine the incidence of mycotoxins in soft cheese and the experiments were conducted to determine qualitatively and quantitatively detection of aflatoxin Ml,quantitative detection of ochratoxin A and isolation and identification of mould contaminant. Of the 50 fresh soft cheese samples tested 8 (32%) and 12 (48%) were found out to contain aflatoxin M 1 for (Kareish and Damietta) respectively, while through usage of lateral flow test ( strip field test). For further confirmation of results by usage of immunoaffinity columns with flurometric assay revealed presence of aflatoxin Ml in mean concentration of 3.6 ± 0.6 (ppb) for kareish cheese samples and 6.7 ± 1.35 (ppb) for Damietta cheese samples. Out of 12 randomly chosen kareish cheese samples undergone detection by immunoaffinity columns with flummetric assay for ochratoxin A presence revealed mean concentration of 0.63 ± 0.48 (ppb) also, 12 randomly chosen Damietta cheese samples revealedmean concentration of2.1 ± 0.35 (ppb). Forty four percent (44%) of kareish cheese tested samples were contaminated with mould the high level of contamination was 1.5 <I 03; the low level was 10 and the mean value was 2.54x 102 ± 6.61 x 10 c f u / gram. |