الفهرس 
Diabetes MellitusOnly 14 pages are availabe for public view
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Abstract
The increased prevalence of T1DM in children and adolescents with its complications especially microvascular ones pushing us to search how to stop or at least postpone the progression of the disease and its complications.
So the aim of the present study was to find out the relation between oxidative stress and adhesion molecules derived from endothelial/platelet activation and involved in the process of endothelial dysfunction and microangiopathy.
This study comprised 38 T1DM children and adolescents following up in the Pediatric Diabetes Clinic, Children’s Hospital, Ain Shams University. They were subdivided into 2 groups according to disease duration.
Group I: comprised 22 patients with disease duration less than 5 years.
Group II: comprised 16 patients with disease duration more than 5 years.
The study included also 15 age and sex matched healthy children and adolescents as controls.
All patients and controls were subjected to the following:-
Careful history taking lying stress up on: Age, sex, age at onset of diabetes, disease duration, Type and dose of insulin treatment, degree of compliance to treatment and any complaint suggestive of microvascular complications.
Full clinical examination with special emphasis on: Anthropometric measurements (weight and height, BMI was calculated), blood pressure, neurological examination for detection of diabetic neuropathy and fundus examination for detection of diabetic retinopathy.
Laboratory investigations included:
• Random blood glucose with calculation of the mean value over the last year prior to the study.
• HbA1c, mean value over the last year was calculated.
• Micro albumin in urine for detection of diabetic nephropathy.
• Lipid profile assay (serum total cholesterol, triglycerides, LDL and HDL. ( using enzymatic colorimetric method.
• Lipid peroxidation (Malondialdehyde) assay by colorimetric method.
• Serum soluble P-selectin assessment by ELIZA technique.
This study revealed the following results:-
Highly significant increase in Malondialdehyde (MDA) level was detected in diabetic groups compared to controls, with the highest level in those patients with longer disease duration (more than 5 years) (P<0.01).
Highly significant increase in serum P-selectin level was detected in diabetic groups compared to controls, with the highest level in those patients with longer disease duration (P<0.01).
Serum MDA level was strongly correlated with serum P-selectin level in diabetics (r=0.91, p<0.0001).
Serum MDA level was positively correlated with disease duration, mean random blood glucose, HbA1c, mean insulin dose, diastolic blood pressure, cholesterol, triglycerides and LDL but negatively correlated with HDL in diabetics.
Serum P-selectin was positively correlated with disease duration, mean random blood glucose, HbA1c, mean insulin dose, diastolic blood pressure, cholesterol, triglycerides and LDL but negatively correlated with HDL in diabetics.
Multiple regression analysis revealed that of all studied variables, HbA1c had the strongest effect on both MDA and P-selectin levels in diabetics.
Both MDA and P-selectin were significantly increased in complicated patients compared to non-complicated, with more increase in those patients with more than one MVC