الفهرس 
acknowlegmentOnly 14 pages are availabe for public view
 |  | from | 228 |  |  |
| | | from | 228 | | |
Abstract
Literature survey revealed that Tipuana Speciosa Benth. and Bauhinia purpurea L. are common ornamental trees in Egypt.
Genus Tipuana has a history of traditional medicinal uses as an emollient in cases including wounds and rheumatic pains. Also, it is used for dysentery, gastritis, hemorrhoids and for uterine inflammation.
While, Genus Bauhinia used as anthelmintic, carminative, prescribed in goiter, in menstruation trouble, for snake bites, scorpion stings and as an antidiabetic herbal remedy.
These activities may be attributed to the presence of a wide array of biologically active secondary metabolites including flavonoids, saponins, coumarins and phenolic acids.
No much work has been carried out on genus Tipuana and flowers of Bauhinia purpurea. Therefore, it was deemed necessary to investigate these plants using leaves and legumes of Tipuana speciosa Benth. and flowers of Bauhinia purpurea L., Fabaceae.
This study includes the isolation and identification of different components of the chosen plants, using different physical, chemical and spectral methods. Study of the biological activities of selected extracts and fractions of T. Speciosa Benth. and B. purpurea L. has been carried out. Plant Tissue Culture technique (P.T.C.) has been utilized to produce some of the biologically active compounds in larger yield than that present in the parent plant. Success in using P.T. C. as an alternative method for production of important secondary metabolites is needed to avoid destruction of the ornamental trees.
This thesis consists of three parts:
Part І
Phytochemical Investigation of Tipuana speciosa Benth. and Bauhinia purpurea L., Family Fabaceae
Chapter 1: Phytochemical Investigation of Tipuana speciosa Benth., Family Fabaceae
Fresh chopped leaves and legumes of T. speciosa Benth. were exhaustively extracted with 70% ethanol by percolation. The ethanolic extracts were filtered, concentrated and fractionated by successive extraction with petroleum ether, chloroform, ether (legumes), ethyl acetate and n-butanol.
The phytochemical investigation of T. speciosa Benth. leaves resulted in the isolation of six crystalline compounds from the different fractions of the plant. On the other hand, two crystalline compounds were isolated from legumes of T. speciosa Benth. β-sitosterol was isolated from the petroleum ether fraction of T. speciosa Benth. leaves. In addition, the ethyl acetate fraction of the same organ yielded nicotiflorine (compound T1) and rutin (compound T2). 4-methoxy-5, 9, 10,10a, 11, 12-hexahydro-[1, 3]dioxolo[4ꞌ, 5ꞌ:4, 5]benzo[1, 2-e]pyrido[1, 2-a]azepin-9-ol (compound T3), soyasaponin ІII (compound T4) and soyasaponin І (compound T5) were isolated from the n-butanol fraction of the same organ. Meanwhile, the n-butanol fraction of legumes of T. speciosa Benth. afforded 3-O-[ β -D- glucopyranosyl ] cholest-5-en-3β-yl (compound T6) and kaempferol-3-O-[β-D-xylopyranosyl (1ꞌꞌꞌꞌꞌ-4ꞌꞌꞌꞌ)-O-α-L-rhamnopyranosyl (1ꞌꞌꞌꞌ-2ꞌꞌꞌ)-O- α-L-rhamnopyranosyl (1ꞌꞌꞌ-2ꞌꞌ)-O-(4’’ acetyl - β-D xylopyranoside ] (compound T7). Structure elucidation of the isolated compounds was carried out using different spectroscopic techniques.