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Abstract In this study 100 samples (50 cow’s milk & 50 fecal swabs) were examined for the presence of E. coli by using conventional method and PCR. E. coli could be isolated from cow’s milk and fecal swabs in a percentage of 18% and 42% respectively. E. coli isolates were tested for susceptibility to eleven antibiotics Ampicillin, Amoxicillin, Amoxicllin/clavulanic acid, Oxytetracycline, Tetracycline, Doxycycline, Ciprofloxacin, Enrofloxacin, Neomycin, Gentamicin and Erythromycin. The resistance to such antibiotics was 50%, 50%, 7%, 67%, 67%, 40%, 23%, 23%, 23%, 13%, and 100% respectively. E. coli isolates were tested for MIC and the results were 60%, 33.5%, 7% for Oxytetracyclin, Enrofloxacin and Amoxicllin/clavvulanic acid respectively for the resistant isolates. Then, the milk samples were examined for presence of antibiotic residues (Oxytetracyclin, Enrofloxacin and Amoxicllin/clavulanic acid) by using chromatographic and serological methods, the percent of positive samples for presence of such antibiotics were 56%, 80% and 64% respectively. Statistical analysis was done to study the relation between Antimicrobial resistance and antibiotic residues. The overall results showed increased resistance percent in isolates from samples with detectable level of antibiotic residues. |