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Abstract This study compared the antibacterial and antifungal effect of chlorhexidine containing gutta - percha ( Activ Ponts ) to calcium hydroxide containing gutta - percha (Calcium Hydroxide Plus ) , both in vivo and in vitro . The first part ( in vitro study ) , a total of forty freshly extracted , single rooted , intact , caries free , human mandibular premolars were collected. |