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العنوان
STUDIES ON SOME ACCLIMATIZATION METHODS OF IN-VITRO PROPAGATED PLANTLETS OF TWO DRY DATE PALM SAKKUTY AND BARTAMODDA CVS\
الناشر
Ain Shams university.
المؤلف
MALHAT,MERVAT HASSAN MOHAMED.
هيئة الاعداد
مشرف / Abdel Moneem El-Bana
مشرف / Ahmed Abd El-Hamid
مشرف / Hassan Mohamed Fadhel El –Wakeel
باحث / MERVAT HASSAN MOHAMED MALHAT
الموضوع
ACCLIMATIZATION. PLANTLETS. TWO DRY DATE PALM. ACCLIMATIZATION. PLANTLETS. TWO DRY DATE PALM.
تاريخ النشر
2012
عدد الصفحات
p.:1410
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البساتين
تاريخ الإجازة
1/1/2012
مكان الإجازة
جامعة عين شمس - كلية الزراعة - Horticulture
الفهرس
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Abstract

This study was conducted at Tissue Culture Lab of Research Laboratory of Date Palm Developing, Giza, Egypt and Egyptian Holland Tissue Culture Company Lab during the period -#102;-#114;-#111;-#109; 2007-2011. The investigation aimed to study in-vitro rooting and to improve survival percentage of Sakkuty and Bartamodda date palm plantlets derived -#102;-#114;-#111;-#109; tissue culture technique. br However, this study was performed through two main experimental groups: br First group – In-vitro rooting experiments: br 1. Effect of gelling agent (Agar-Gelrite). br 2. Effect of sucrose and activated charcoal. br 3. Effect of Poly Ethylene glycol (PEG.( br 4. Effect of light Intensity. br Second group – In-vivo acclimatization experiments: br 1. Effect of Antitranspiration agent in green house. br 2. Effect of planting medium. br The main results could be summarized as follows: br I. In-vitro rooting experiments: br 1. Effect of some gelling agent: Gelrite at 2 g/L and agar at 6g/L both with Sakkouty gave higher rooting percentage . Gelrite at 4 and 3 g/L with Sakkouty recorded the highest significant average number of roots/shoot. Agar at 8 g/L with Bartamodda recorded the highest significant root length. The higher survival percentage was found by treatments of Agar at 6 g/L with Sakkouty and gelrite at 3g/L with Sakkouty than that of agar at 10 g/L with Bartamodda. br 2. Effect of sucrose and activated charcoal: Sucrose, at 45g/L with or without activated charcoal recorded the highest significant rooting percentage. Absence of activated charcoal with Bartamodda gave higher rooting percentage than that of activated charcoal with Sakkouty. br The highest average number of roots/shoot was found by treatment, sucrose at 45g/L, Sakkouty with activated charcoal. br Average root length showed the highest significant value by treatment of sucrose at 45gm/L, Bartamodda and with activated charcoal. br Out survival percentage during acclimatization stage gave insignificant differences among treatments. br 3. Effect of Poly Ethylene glycol (PEG): The greatest plantlet length (cm) during in-vitro rooting stage was found by treatments of PEG at 0.0 g/L and 5 g/L with Sakkouty than most of other treatments. br Higher significant number of roots/shoot was recorded with treatment of, PEG at 0.0 g/L with Sakkouty compared with most of other treatments. br Average root length, showed insignificant differences among treatments. br Survival percentage showed higher significant values with treatments of, PEG at 15g/L with Sakkouty and Bartamodda than that of control of both cvs. br The highest plant length (cm) during acclimatization stage was found by treatment of PEG at 0.0g/L with Sakkuty than most of other treatments. br The greater number of leaves/plant during acclimatization stage was found by PEG at 5.0g/L with Sakkouty than 20g/L with Bartamodda. br 4. Effect of light Intensity: Average root length (cm), plantlet length and number of roots/shoot during in-vitro rooting stage were the higher significant at 3000 lux level with Bartamodda than that of 9000 lux with Sakkouty. br Survival percentage, plant length and leaf number per plant during out acclimatization stage showed insignificant differences among treatments. br II. In-vivo acclimatization experiments: br 1. Effect of antitranspiration: Survival percentage had insignificant differences among antitranspiration treatments. br Plant length showed higher significant values with 0.5 ml/L with both cvs. than most of other treatments. br Number of leaves per plant had higher significant value at 0.5 ml/L with Bartamodda than some of other treatments. br 2. Effect of planting medium: br The higher significant value of survival percentage was found by treatment of the third medium [Vermiculite+Perlite +Peatmoss( 1 :2 :1 v/v)] with Sakkouty than some of other treatments . br Number of leaves per plant had insignificant differences among treatments. br Higher significant plant length was found by the first medium [Vermiculite+Perlite +Peatmoss( 1 :1 :1 v/v) ] with Sakkouty than some of other treatments.