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Abstract Hiochermcal and serological charactenzation to isolated bacteria was encountered. This study included 90 poultry carcasses, products and environmental samples {( 10 environmental samples, 3l\ poultry meat samples and 42 poultry products sample which included ( 11 liver, 19 luncheon , 9 gizzard , 3 heart samples )} were collected from different markets at Cairo and Menoufia Governorates. The samples were transported in ice tank to laboratory as soon as possible and culture on selective media. The isolated bacteria were identified by growth characters, motility, and biochemical reaction and serotyping using specific antisera. Results of investigation revealed that Salmonella species were detected in environmental samples (utensils, water), poultry carcasses and poultry products (gizzard, liver, luncheon and heart) at a percentage of 10%, 2.6% and 2.3% respectively. Out of these positive salmonella isolates (66.6%) strains were belonged to Salmonella enteritidis and 33.3%. strains were belonged to S. typhimurium. The results of isolation of different bacteria from poultry products indicate that Proteus vulgaris and P. mirabilis were the most isolated species from poultry products with a percentage of (14.3% for each ) followed by Serratia marcescens , Staphylococcus aureus , Morganella morganii . E coli . Shigella dysenteriae and S. enteritidis wr.,tn a percentage or~ I•• I. Q/, 95. ,I.~l ,’J~.8 and7~4.°’ 70 respecti.v. ely Results of bacteria isolated from local luncheon samples indicated that the most prevalent species were Proteus vulgaris, Serratia marcescens, Enterobacter aerogenes, Pseudomonas aeruginosa, Enterococcus faecalis and Klehsielfa pneumoniae (11.1 ~/o for each) , followed by Proteus inirabilis, Proteus morganii , Serratia liquefaciens , E. coli , S. aureus and Shigella dysentery with a percentage of 5.5% each. Meanwhile, results of bacteria isolated from liver samples revealed that Proteus mirabilis was the most prevalent species (33.3%) followed by Serratia marcescens and Enterobacter aerogenes with the percentage of (20% each ) while Serratia liquefaciens, E coli. S aureus and Klebsiella pneumoniae were isolated with a percentage of 6.6 %for each. Results of bacteria isolated from gizzard samples showed that Proteus vulgaris was the most prevalent one (30%) followed by P. morganii (20%) while S. enteritidis, S. aureus. Shigella dysenteriae, Enterococcus faecalis, Pneumococci were isolated with a percentage of 10% each. Results of bacteria isolated from heart samples it was found that Pseudomonas aeruginosa , S. aureus and P. vulgaris were isolated with a percentage of333% for each. Concerning environmental samples (water -utensils and poultry processing plant), results revealed that 10 environmental samples of water and utensils in processing plant harbouring bacteria with a total number of 9 isolates. Shigella dysentenae. Enterobacter hafniae, S. aureus were isolated from water with percentage of 33.3% while Enterobacter agglomcrans. Serratia niarcesccns. Serratia liquefacicns. Proteus mirabilis. Klebsiella pneumoniac and Salmonella enteritidis wvv\e..-re\,.:J._isolat\edl, fIro\.}mI utl’evnII;s’)i1ls• W’I”till, ,UJ,Iv~ ’f--lV”l”lv~l.,;”1ld1bl’.’,’;’’~’oI f I16..6°//0. Re-s’.uUlts;’) ’0.+I bacteria isolated from tissue samples of poultry carcasses revealed Enterobacter agglomerans was the most prevalent species with the percentage of (13.5%) followed by Enterobacter cloacae. Serratia marcescens. Klebsiella pneumoniae. P vulgaris. Shigella dysenteriae, A1urganella morganu. Ente rn btu tr> r • .......-’/ ••• J ••..• aerogenes. s. aureus. Pseudomonas aerugtnosa. Serratia liquefaciens . |