الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 105 |  |  |
| | | from | 105 | | |
Abstract
Poultry industry in A.R.E. was subjected to various problems. One of them was the early chick mortalities that causes great economical losses and its investigation is the request of poultry Scientists and Poulry men. So this study was planned as an attempt to throw spot lights on the following items: 1-Exploration of the possible bacterial causitive agents of early chick mortalities Sharkia Governorate. 2-Morphological, biochemical and serological identifica-tion of the isolated microorganisms. And testing of their antibiotics sensitivity in vitro to choose the highly potent ones recauminded to save guard such creatures from early deaths. For attaining this goal a total of one hundred and fity baby chicks died within the first ten days of their life were bacteriologically examined in a trial to detect of the actual bacterial causes of death. Such samples were randomly collected from both governmental and private poultry farms in various localities in Sharkia Governorate viz: 35 samples were collected from Anshas, 30 samples were collected from Minia Al-Kameh, 15 samples were collected from Abou-Hammad, 50 samples were collected from Belbias besides 20 samples were collected from Zagazig City. These samples were subjected to bacteriological examination to explore the actual bacterial causative agents. The obtained data revealed that 42 isolates were E. coli, their serological typing revealed that (13 of 0125:B76, 8 of 0119:B69, 5 of 078:B80, 4 of 055:B59 and 12 still untyped, 45 isolates were Salmonella spp. theirserological typing revealed that [21 out of them were S. pullorum), (9) were S. gallinarum, 7 were S. typhimurium and 8 still untyped], 27 isolates belonged to Staph. aureus, 6 isolates were Kl. pneumoniae and only 3 isolates were Pseudomonas aeruginosa with an incidence of 28%, 30%, 27%, 4% and 2% respectively. A disc diffusion technique was done to study the antibiotic sensitivity pattern of these causative agents in vitro by using twelve antibiotic discs viz: Ampicillin (10 pg BioMerieux) chloramphenicol (30 pg bioMerieux), colistin sulphate (10 pg BioMerieux) erythromycin (15 pg BioMerieux), neomycin (30 pg BioMerieux), nitrofurantoin (300 pg BioMerieux), gentamicin (10 pg Oxoid), nalidixic acid (30 pg Oxoid), doxycycline (30 pg Oxoid), streptomycin (10 pg Pasteur Lab.), flumequine (30 pg BioMerieux), and lincospectin (50-100 pg Pasteur Lab.). The activity percentage of each antibiotic disc which is the ratio between the sensitive strains to the totally tested ones was calculated.