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Abstract Since fish and shellfish are subjected to the risk of contamination with various pathogenes either during their presence in their aquatic environments or after being harvested for marketing. This work was done to assess sanitary condition of Tilalpia nilotica and Clarias lazera which are the common type of fish in Egypt taken from urban and rural markets.One hundred samples of Tilapia nilotica and Clarias lazera Taken from urban and rural markets were examined organolepticaly, Bacteriologicaly. Mycologically and chemic ally. The organoleptic examination for the samples revealed that all samples can be considered fresh, the overall mean The Bacteriological examination revealed that the total colony count from surface of Tilalpia nilatica and Clarias lazera taken from urban and rural area in an overall mean were (52.1 x 10 پ} 50 x 10 ) lower count recorded from urban areas of Tilpia nilotica and Clarias lazera than those of rural areas Muscle total colony count revealed that Tilpia nilotica of rural areas recorded higher count than those of urban areas (890.4 } 63.73 , 783.2+ 60.74 x 10), respectively Bacteria belonging to the following genera could be isolated from the surface , muscle and intestine each of Tilapia nilotica and Clarias lazera from urban and rural areas in different percentages~ Escherichia, Salmonella, Citrobacter, Enterobacter, Klebsiella, Proteus, Serratia, Shigella, Pseudomonas. Flavobacterium, Achromobacter, Chrom— obacterium, A)(a.ligenes, Aeromonas, Staphylococcus aureus and S.epidermidis. Concerining the mould and yeast the following genera could be isolated in different percentages from the surface of Tilapia niloticna and Clarias lazera from urban and rural areas; Aspergillus, Penicillium, Cladosporium, Trichosporium, Thamnidium Fusarium, Mucor, Trichoderma, and Candida, Torulopsis. Sacchromyces. and Rhodotorula for yeasts. The chemical examination revealed that the PH values of Tilapia nilotica from urban and rural areas The presence of large number of total colony count total Enterobacteriacea count, total Coliform count and total Staphylococcus count in the examined samples indicates that the fish loaded with these microorganisms from the polluted water of the rivers and lakes which may be polluted form sewage disposal and after catching during unsanitary handling and marketing. The public health impotance of the isolated microorganisms waS discussed. |