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Abstract Annout catfish were maintained at optimal environmental conditions where ~the water temperature adjusted at 22°C, the fish diet was offered daily at the rate of 1% of the total biomass and the stocking density was 15,0 g /Ld had a vagorous antibody response to Aeromonas~strain These antibodies were detected in the serum of infected fish from the first week with good amount and reached to its maximum concentration at the end of the taurth week, The low water temperature that was adjusted at (13 پ} 1°C) possessed a direct imunosuppression effect on the fish maintained at this temperature, As they had a poor antibody response incomparison with, the vigorous antibody response of the control group and the maximum titer reached at the end of the third week post~inoculatioh, In contrast , the fish maintained at high water temperature (28°C) had a vigorous antibody response to great extent similar to the control Fish fasted for 2 weeks proir to injection of Aeromonas ~2≥ila strain and for the duration of the study and ma~~ ntaiiaed under environmental condition virtually identical to the controls had antibody response not significantly different from those of the controls Fish also maintained under crowded conditions (75o0 g of body mass/b) had antibody response not significantly different from those of the controls Fish exposed to combined environmental conditions had immune responses to some or great extent different from those of the controls This defferences depend upon the presence or obsence of low water temperature which is the only factor enable to induce Immune suppression in Anout catfish exprimentally injected with Aeromonas~ antigen0 3.— Fish exposed to fasting with low water temperature (13 پ} 1°C) had a poor antibody response inoomparison with the vigorous antibody response of the control group and the maximum titer reaohed at the end of the thi~ rd week, In contrast ~ the fish exposed to fasting and high water temperature (28°C) had immune response to some extent different from those of the controls • 2~ Fish exposed to overcrowding (75~O g of body rnass/L ) with temperature (13 پ} 1°C) had immune responses to great extent differed from those of the control group and the maximum titer reached at the end of the third week In contrast9 the fish exposed to overcrowding with high water temperature (28°C) had antibody response to some extent different from those of the controls 3~ :?i2h exposed to fasting and overarowaing bad antibody response to some extent different from those of the controls 4~ Fish exposed to fasting9 overorowdixig and low water temperature (13 پ} 100) had antibody response to great extent different from those of the control group and the maximum titer reached at the end of the third week0 In contrast9 the fish exposed to fasting ~ ove overcrowding and high water temperature (28°C) had antibody response to some extent diffe~ent from those of the controls The antibody response was evaluated by microagglutjn ation test and passive heamagglutinatjon test0 The compar.~ ison between the antibody titer evaluated by each test indicates that~ the passive hea~agg]attmnatj~~ is more sensitive than micro~glutinatjon for the detection of antibody in the serum. |