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Abstract A total of 146 samples of luncheon (50), basterma (27), stool from diarrhoeal children (33) and control children (36) cases with “no complain” of diarrhoea at age range from 6 to 12 years old (whome case history was that they ate lunchoen and/or basterma at least the day before stool specimens collection), were collected from Gin, Cairo and Kalyobia Governorates and examined bacteriologically for isolation of E.coli, including the different pathogenic E.coli groups. The incidence of E.coli in luncheon and basterma was 18% and 11.1%, respectively, while the incidence of pathogenic E.colj organisms in luncheon, basterma, diarrhoeal and control children stool specimens was 8%; 3.7%; 39.4 and 33.3%, respectively. The serological identification of the isolated E.coli revealed that out of 124 E.coli strains isolated from the examined samples, 52 strains could be serotyped and belonged to 15 different pathogenic E.coli serovars. These 0 serovars were 06, OlSabOlBac, 025, 026, 028ab028ac, 044, 078, 086, 0111, Oll2abOll2ac, 0114, 0119, 0124, 0126 and 0128, those could be categorized into 4 different pathogenic E.coli groups including enteropathogenic Eseherichia ccli (EPEC); enterotoxigenic E.coli (ETEC), enterohemorrhagic E.coli (EHEC); and enterojnvasive E.coli (EIEC). The incidence of enteropathogenic E.coli (EPEC) serovars in luncheon, diarrhoeal and control children cases was 6%, 30.3% and 19.4%, respectively. The incidence of enterotoxigenic E.coli (ETEC) serovars in luncheon, basterma, diarrhoeal and control children stool specimens was 6%, 3.7%, 15.2%. and 16,7%, respectively. While the incidence of enterohemorrhagic E.coli (EHEC) serovars in luncheon and diarrhoeal children stool specimens was 4% and 3%, respectively; however, the incidence of enteroinvasive E.coli (EIEC) serovars in stool of diarrhoeal and control children was 6.1% and 5.6%, respectively. Nearly all the isolated pathogenic E.coli serovars isolated from the examined ready-to-eat meat products could be also isolated from stool of children suffering from diarrhoea suggesting the possibility of transmission of these pathogenic E.coli to children through the consumption of contaminated ready-to-eat meat products (Luncheon and Basterma). The results revealed that the most prevalent 0 serovars isolated from the examined ready-to-eat meat products were 078, OlSabOlBac and 0128, however, the most prevalent 0 serovars isoalted from diarrhoeal children stool specimens were 0128, 025 and 078, while 0128 and 078 were the most prevalent one isolated from stool of control children. The enterotoxigenic activity of the isolated E.coli that produced enterotoxin(s) was belonged to 5 serovars (Ol8ab Ol8ac, O2BabO2Sac, 044, 078 and 0128) and also some of untypable E.coli were LT producers. Out of 26 E.coli strains isolated from luncheon, 5(19.2%) produced LT; 2 (7.7%) produced ST and 1(3.8%) produced both LT and ST. While out of 11 E.coli strains isolated from basterma, only one (9.1 %) was LT producer. On the other hand, out of 47 E.coli strains isolated from diarrhoeal children 10(21.3 %) produced LT; 4(8.5 %) produced ST and 3(6.4 %) produced both LT and ST. While out of 40 E.colj strains, isolated from control children, only one (2.5 %) produced LT but non produced ST nor LT + ST. The antibiotic sensitivity test of the isolated E.coli was done using 13 different antibiotics. The incidence of antibiotic resistant E.coli against ampicillin, flumequine, chloramphenicol, carbenicillin, doxycycline, erythromydin, gentamicin, kanamycin, nalidixic acid, novobiocin, spira mycin, trimethoprim/sulfamethoxazole and oxytetracycline was 97.6%; 0%; 42%; 59.7%; 92.7%; 94.4%; 43.5%; 23.4%; 21%; 90.3%; 96.8%; 54.8% and 100%, respectively. It is worth mentioning that the untypable E.coli showed more antibiotic resistant to ampicillin , chloramphenicol, carbenicillin kanamycin , nalidixic acid, spiramycin and trimethoprim! sulfamethoxazole than the typable pathogenic E.coli. Moreover / flumequine was considered the most effective antibiotic against the E.coli strains isolated in the current study. The hygienic importance of the isolated pathogenic E.coli was discussed. |