الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
In the present study, 230 swabs (fecal, dust, drag, litter, drooping) and hatched eggs and hatched chicks were collected from broiler breeder farms to investigate the prevalence of Salmonella species.The isolation of Salmonella revealed 20 Salmonella suspected positive samples. Biochemical identification by several chemical tests as well as API confirmed the Salmonella species identification. Antimicrobial sensitivity testing against 13 antibiotic agents revealed that (95%) of the isolates were sensitive to oxytetracycline followed by cefotaxime (80%) then ampicillin (75%) and chloramphenicol (65%), whereas, the others ranged from 55% to 50%. Also, 95% of the isolates were resistant to penicillin, 85% to norfloxacine and 75% to gentamicin and others showed lower percent of resistance. 2 pairs of specific primers to amplify fragments of fliC and sefA genes of Salmonella were used to confirm the molecular characterization of Salmonella Kentucky, S. Enteritidis and S. Typhimurium in the isolated strains in genotyping PCR assay. Sequence analysis of the amplified PCR products for Salmonella Kentucky, Enteritidis and Typhimurium were carried out and phylogenetic analysis of the obtained sequences revealed the close identity of the isolated strains with other Salmonella reference strains in different countries. Indeed, the present study reports the circulation of Salmonella Kentucky, Enteritidis and Typhimurium among broiler breeder farms in Egypt.